We narrowed to 360 results for: %s

1. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

   Type

   Blog Post

   Published

   June 22, 2017, 2:30 p.m.

   ...PubMed Central PMCID: PMC3074624. 5. Bindels, Daphne S., et al. "mScarlet: a bright monomeric red fluorescent...

2. Google Forums Round Up: First Impressions of NgAgo

   Type

   Blog Post

   Published

   Aug. 4, 2016, 2:30 p.m.

   ...DNA-scissors (blog). Blog website. 4. Hsu, Patrick D., Eric S. Lander, and Feng Zhang. "Development and applications...

3. Your Lentiviral Plasmid FAQs Answered

   Type

   Blog Post

   Published

   April 23, 2014, 1:08 p.m.

   ...Many transfer vectors are SIN. Packaging plasmid(s) contain the minimal number of HIV genes required ...

4. New Tools Enable CRISPRa for Neuroscience Applications

   Type

   Blog Post

   Published

   July 2, 2019, 12:51 p.m.

   ...lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with neuron-specific expression...

5. Isolating Genomic Regions of Interest with the CRISPR System

   Type

   Blog Post

   Published

   May 30, 2019, 12:44 p.m.

   ...(2017): 1028-1043. PubMed PMID: 28841410. Qi, Lei S., et al. "Repurposing CRISPR as an RNA-guided platform...

6. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

   Type

   Blog Post

   Published

   July 16, 2020, 1:15 p.m.

   ...broadly one can interpret our analysis of COVID-19’s effect on scientific activity. The effects of COVID...

7. DNA Purification Without a Kit

   Type

   Blog Post

   Published

   Feb. 11, 2020, 2:15 p.m.

   ... Y., He, W., Wang, J., Peng, F., Huang, L., Zhao, S., & Deng, W. (2018). Rapid Regeneration and Reuse ...

8. Viral Vectors 101: Preparing Pooled Libraries

   Type

   Blog Post

   Published

   July 13, 2023, 1:15 p.m.

   ...10.1002/cpmb.8. PMID: 27366891. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD...

9. Getting to Know Expansion Microscopy

   Type

   Blog Post

   Published

   June 20, 2023, 1:15 p.m.

   ...992. https://doi.org/10.1038/nbt.3625  Truckenbrodt S, Sommer C, Rizzoli SO, Danzl JG (2019) A practical...

10. Bioinformatics at Addgene

    Type

    Blog Post

    Published

    July 14, 2022, 1:15 p.m.

    ...sequential computational tasks for which existing script(s) have been developed for a singular purpose (ex. adapter...

11. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ...Kadina, Adrianna E. Zepeda, Kevin Holden, Jonathan S. Gootenberg, Omar O. Abudayyeh. Drag-and-drop genome...

12. Plasmids 101: Shuttle Vectors

    Type

    Blog Post

    Published

    May 20, 2025, 1:15 p.m.

    ... can be used across species, for example, in both S. cerevisiae and E. coli. Auxotrophic selection is ...

13. Plasmids 101: Codon usage bias

    Type

    Blog Post

    Published

    Sept. 27, 2018, 1:09 p.m.

    ...well correlated with codon bias in both E. coli and S. cerevisiae. Protein folding - If a protein is encoded...

14. AAVs in Retinal Gene Therapy

    Type

    Blog Post

    Published

    July 31, 2018, 12:55 p.m.

    ...dystrophy)? AAV gene therapy started in the mid-90's with a lot of work and a little bit of luck A pioneer...

15. Hot Plasmids - November 2023

    Type

    Blog Post

    Published

    Nov. 14, 2023, 3:35 a.m.

    ... Find PE6 plasmids here!  Doman, J.L., Pandey, S., et al. (2023). Phage-assisted evolution and protein...

16. How to Be an Excellent Trainee

    Type

    Blog Post

    Published

    Sept. 26, 2023, 1:15 p.m.

    ...days or weeks), look over your notes and protocol(s). Is there enough information there for you to be ...

17. Great Results Start with Great Standard Curves

    Type

    Blog Post

    Published

    Aug. 15, 2024, 1:15 p.m.

    ... standard, standard dilutions, and unknown sample(s) in the same buffer if possible, or very similar buffers...

18. Analyzing CRISPR Editing Results with ICE from Synthego

    Type

    Blog Post

    Published

    May 8, 2018, 1 p.m.

    ...traces in the region around the guide binding site(s). The sequence base calls from the .ab1 file are also...

19. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    Published

    Feb. 27, 2018, 2:04 p.m.

    ...Pleiner, T., Schliep, J.E., Stark, H., Trakhanov, S., & Urlaub, H. (2015). Nanobodies: site-specific labeling...

20. Transferable Skills Guide: Career Planning Resources

    Type

    Blog Post

    Published

    Nov. 14, 2017, 2 p.m.

    ...20140417174121-34888774-linkedin-etiquette-guide-20-do-s-don-ts Resources for getting into grad school: https...