We narrowed to 360 results for: %s

1. AAVs CREATed for Gene Delivery to the CNS and PNS

   Type

   Blog Post

   Published

   Sept. 28, 2017, 2:01 p.m.

   ...the pre-designed primers to determine which version(s) of the capsid gene successfully infected the cell...

2. Fluorescent Proteins 101: Fluorescent Protein Timers

   Type

   Blog Post

   Published

   May 4, 2017, 2:30 p.m.

   .... 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation revealed...

3. Fluorescent Proteins 101: Aptamer Fluorophores

   Type

   Blog Post

   Published

   April 11, 2017, 2:30 p.m.

   ...Nature Methods 9, 787–790 (2012). 3. Filonov, Gregory S., et al. “In-gel imaging of RNA processing using broccoli...

4. MXS Chaining

   Type

   Blog Post

   Published

   Feb. 7, 2017, 3:30 p.m.

   ...: PMC2822740. 2. Engler C, Kandzia R, Marillonnet S. A one pot, one step, precision cloning method with...

5. Plasmids 101: Gateway Cloning

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   Blog Post

   Published

   Jan. 12, 2017, 3:30 p.m.

   ...attL-entry-TOPO vector. TOPO cloning adds short end(s) to facilitate cloning into an attL-containing entry...

6. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

   Type

   Blog Post

   Published

   Aug. 9, 2016, 2:30 p.m.

   ... --very-sensitive-local -L 9 -N 1 -U GRNA_23MERS -S GRNA_HITS.sam Many thanks to our guest blogger, Aneesh...

7. Plasmids 101: Control Plasmids

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   Blog Post

   Published

   April 9, 2015, 3:29 p.m.

   ...decreased expression of Gene X. Ergo the positive control(s) should decrease the expression of Gene X. Since the...

8. Tips for Using FRET in Your Experiments

   Type

   Blog Post

   Published

   Nov. 5, 2014, 3:41 p.m.

   .... 2007 Sep; 32(9): 407-14. Zimmermann T,  Terjung S. FRET Basics and Applications: An EAMNET Teaching ...

9. Plasmids 101: The Promoter Region – Let's Go!

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   Blog Post

   Published

   April 3, 2014, 8:05 p.m.

   ...interest will be expressed.      The RNA polymerase(s) RNA is transcribed from DNA using an RNA polymerase...

10. Negotiating Work and Life: How to Find the Joy

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    Blog Post

    Published

    March 20, 2014, 3:04 p.m.

    ... not to do this. For a family with working parent(s), the sooner your kids can make a decent meal, fold...

11. Troubleshooting and Optimizing a Western Blot

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    Blog Post

    Published

    Sept. 17, 2024, 1:15 p.m.

    ...trouble by confirming your transfer worked with Ponceau S staining or other reversible protein staining immediately...resources References Luo, H., Rankin, G., Straley, S., & Chen, Y. (2011). Prolonged Incubation and Stacked...

12. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type

    Blog Post

    Published

    July 7, 2020, 1:15 p.m.

    ...Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow LE (2020) GO: a functional reporter system to...

13. Transferable Skills Guide: Creativity

    Type

    Blog Post

    Published

    Jan. 18, 2022, 2:15 p.m.

    ...mental-mistakes-that-kill-your-productivity Finkelstein, S. (2019, July 15). A brief exercise to spur innovation...

14. QC Sequencing Technologies at Addgene

    Type

    Blog Post

    Published

    July 15, 2025, 1:15 p.m.

    ...//doi.org/10.1021/ac2010857  Sanger, F., Nicklen, S., & Coulson, A. R. (1977). DNA sequencing with chain-terminating...

15. Plasmids 101: Plasmid Incompatibility

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    Blog Post

    Published

    March 31, 2020, 1:15 p.m.

    ...2958.2005.04643.x Pubmed PMID: 15916596 Kamruzzaman M, Shoma S, Thomas CM, Partridge SR, Iredell JR (2017) Plasmid...

16. Hot Plasmids Spring 2024

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    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ...many closely-packed labeled cells. Plus, Voltron2’s fluorescence is tunable by changing the dye that becomes...

17. Changing Labor Laws Bring Increased Postdoc Wages

    Type

    Blog Post

    Published

    Oct. 25, 2016, 2:30 p.m.

    ...Many thanks to our guest blogger Gary McDowell! Gary S. McDowell is currently the Executive Director of Future...

18. Site Directed Mutagenesis by PCR

    Type

    Blog Post

    Published

    Aug. 2, 2016, 2:30 p.m.

    ...minor extension can usually ensure that the 3’-base(s) do not form secondary structures. The introduction...

19. How to Negotiate a Successful Lab Start Up Budget

    Type

    Blog Post

    Published

    Sept. 1, 2015, 2:30 p.m.

    ... tactic, and puts you ahead of most start-up P.I.’s. It doesn’t have to be complicated, or difficult to...

20. 27 Hot Plasmids from 2016

    Type

    Blog Post

    Published

    Dec. 22, 2016, 3:03 p.m.

    ...PMID: 25895059 Barnea, et al. Proc Natl Acad Sci U S A. 2008. PubMed PMID: 18165312 CIDAR MoClo parts ...or clusters. Veggiani, et al. Proc Natl Acad Sci U S A. 2016. PubMed PMID: 26787909 EasyClone 2.0 Yeast...Hanson SM, Rodríguez-Laureano L, Albanese SK, Gradia S, Jeans C, Seeliger MA, Levinson NM, Chodera JD. bioRxiv...