We narrowed to 620 results for: Dos

It's important to determine how much functional virus you have a in your prep before starting a transduction. Learn how to titer your lentivirus here.

March 15, 2016, 2:30 p.m.

Golden gate cloning can be used to efficiently and inexpensively clone multiple inserts into a single vector. Learn the basics of golden gate here.

Aug. 27, 2015, 2:30 p.m.

Tips for writing your scientific manuscript, including how to search for and organize papers.

May 5, 2015, 3:54 p.m.

An introduction to transposons including Sleeping Beauty, piggyBac, and Tol2 and how they can be used in transposon mutagenesis screens, gene transfer, and creating transgenic animals.

July 6, 2021, 1:28 p.m.

Practical advice for using CRISPRs: designing your gRNA and introducing it into cells.

Sept. 24, 2020, 1:15 p.m.

Learn about some of the factors you should take into consideration when choosing the appropriate AAV for your next experiment.

June 13, 2017, 2:30 p.m.

Learn how the Journal of Emerging Investigators enables high school students to learn more about the scientific process and scientific publishing.

Sept. 5, 2018, 12:01 p.m.

There are a few different types of Fab fragments to consider when you need a smaller affinity reagent than antibodies. Learn about them here!

Sept. 5, 2024, 1:15 p.m.

This post explains how to strip and reprobe a western blot, which is useful for detecting multiple targets or for correcting errors.

Aug. 1, 2024, 1:15 p.m.

A review of alternative PAM and PAM-flexible Cas enzymes.

March 7, 2024, 2:39 p.m.

Learn about a variety of AAV encoded calcium biosensors available from Addgene.

Aug. 10, 2023, 1 p.m.

Overview of virus-specific safety controls as well as general safety procedures for viral handling.

June 22, 2023, 1:15 p.m.

ELISA are used to quantitatively detect protein concentrations in experimental samples. Learn about different types of ELISA and when to choose each method.

Aug. 24, 2021, 1:15 p.m.

Find seven tips for scientists when preparing for an industry interview, including advice for remote interviewing.

July 9, 2020, 1:15 p.m.

A toolkit of synthetic promoters for transgene expression in AAV can be used for targeted gene expression in retinal cell types in mice, primate, and human retinas.

March 12, 2020, 1:15 p.m.

This blog posts discusses Cas9 gene drives and the appropriate safeguard and confinement strategies scientists should use for these gene drives.

May 22, 2015, 4:58 p.m.

Western blots have many technical elements. We cover each element, and the different options available to choose from, in this post.

Aug. 22, 2024, 1:15 p.m.

Struggling to get started with designing your first flow panel? This blog walks you through what you need to know and an example panel design to help you get started!

May 28, 2024, 1:45 p.m.

Learn what a high fidelity DNA ligase is and when you might need one.

March 6, 2018, 1:49 p.m.

Get perspectives and possibilities for future action from scientists and other citizens who participated in the March for Science.

Oct. 24, 2017, 1:54 p.m.