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Addgene uses bioinformations to streamline our sequencing and QC workflow. Learn how we built the process in this blog.

July 14, 2022, 1:15 p.m.

Two new CRISPR tools from the Abudayyeh-Gootenberg lab are now available at Addgene: Cas7/11 and PASTE.

Feb. 17, 2022, 2 p.m.

David Liu's lab analyzed how COVID-19 affected their plasmid distribution.

July 16, 2020, 1:15 p.m.

Find three ways to purify genomic DNA, plasmid DNA, or PCR products without a commercial kit. Also, find a method for regenerating silica columns for reuse.

Feb. 11, 2020, 2:15 p.m.

Learn about CRISPR activation tools for neuroscience including a SPH transgenic mice, neuron-optimized viral vectors, and high-throughput screening.

July 2, 2019, 12:51 p.m.

Read on to learn more about this technique to isolate specific genomic regions using CRISPR.

May 30, 2019, 12:44 p.m.

Learn how and why Adeno-associated virus (AAV) has enabled breakthroughs in retinal gene therapy.

July 31, 2018, 12:55 p.m.

Learn how you can use Synthego's ICE tool to analyze your CRISPR genome editing experiments.

May 8, 2018, 1 p.m.

Learn about the advantages of secondary nanobodies from the Gorlich lab over traditional antibodies. Applications include one-step fluorescent immunostaining.

Feb. 27, 2018, 2:04 p.m.

Learn about the many resources you can use for career development as a graduate student or post doc.

Nov. 14, 2017, 2 p.m.

Learn how the Gradinaru Lab at Caltech developed AAV vectors to target the peripheral and central nervous systems.

Sept. 28, 2017, 2:01 p.m.

Fluorescent protein timers can be used to determine molecular age. Learn how to use these unique tools in this post.

May 4, 2017, 2:30 p.m.

Learn about aptamer-based fluorphores and how they can be used to brighten up your experiments.

April 11, 2017, 2:30 p.m.

Learn how you can use MXS-Chaining to generate complex, multicistronic plasmid constructs.

Feb. 7, 2017, 3:30 p.m.

Scientists are not limited to restriction enzyme cloning. Gateway cloning is a rapid and efficient way to move DNA into multiple vector systems.

Jan. 12, 2017, 3:30 p.m.

Learn how to design a CRISPR screening experiment targeting enhancers.

Aug. 9, 2016, 2:30 p.m.

The basics of Fluorescence Resonance Energy Transfer (FRET), including tips and parameters to consider when using FRET for your experiments.

Nov. 5, 2014, 3:41 p.m.

Ten tips to help scientists negotiate their work/life balance from Joanne Kamens, Addgene's Executive Director.

March 20, 2014, 3:04 p.m.

A dive into troubleshooting and optimization steps you can take to improve your western blot or identify and fix issues within the assay.

Sept. 17, 2024, 1:15 p.m.

Learn about what sequencing technologies the quality control team at Addgene uses to maintain a high standard of plasmid and viral vector quality.

July 15, 2025, 1:15 p.m.