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Curious about what's new in the Addgene repository this spring? Check out our latest Hot Plasmids post!

May 23, 2024, 1:15 p.m.

Expansion microscopy allows for light microscope imaging of fixed tissues while overcoming the 250 nm resolution limit.

June 20, 2023, 1:15 p.m.

Learn about how to identify and develop creativity in the lab - a skill that's valuable in and out of the sciences!

Jan. 18, 2022, 2:15 p.m.

Use these CRISPR base editing reporters to track base editing events in real-time and enrich for base edited cells.

July 7, 2020, 1:15 p.m.

Plasmid incompatibility is the inability of different plasmids to be maintained in a cell. We’ll cover why this happens and how it might affect your experiment.

March 31, 2020, 1:15 p.m.

Learn why codon usage bias exists and techniques you can use to avoid the detrimental affects of codon usage bias on heterologous protein expression - codon optimization and expression of alternative tRNAs.

Sept. 27, 2018, 1:09 p.m.

Gary McDowell, executive director of Future of Research, describes what labor laws will mean for many postdocs. TLDR - increased salary or overtime tracking.

Oct. 25, 2016, 2:30 p.m.

Use site directed mutagenesis to insert small mutations into your plasmid of interest and follow these tips for a smooth process with easy validation.

Aug. 2, 2016, 2:30 p.m.

A laboratory research management consultant and coach provides step-by-step advice on how to negotiate a budget when stating your lab.

Sept. 1, 2015, 2:30 p.m.

Controls are required for any plasmid-based experiment in order to accurately assess the results. This blog posts reviews different types of control plasmids, including but not limited to internal controls, negative controls, positive controls, and replicate controls.

April 9, 2015, 3:29 p.m.

The next post in the Plasmids 101 series focuses on the promoter region, the element responsible for initiating the transcription of your insert into RNA.

April 3, 2014, 8:05 p.m.

Check out all of the plasmids featured in our hot plasmids articles from 2016.

Dec. 22, 2016, 3:03 p.m.

This post, written by the creator of the CRISPRecise kit, explains how to use it to minimize off-target effects in CRISPR genome editing.

March 31, 2026, 1:15 p.m.

Joining the popular sacB counterselection method is a new suite of toxin-based allelic exchange vector that can be used in many proteobacterial genomes.

Aug. 15, 2019, 12:30 p.m.

Stochastic multicolor labeling is a popular technique to label individual cells with different colors and allows for lineage tracing of cell populations over time. Read this blog post to learn about the different systems for various organisms.

March 5, 2019, 1:08 p.m.

Scientists are currently developing disease treatments using CRISPR. Learn about recent developments in the fight against HIV-1 with CRISPR.

May 10, 2016, 2:30 p.m.

Gibson Assembly is a popular cloning alternative to traditional restriction/ligation methods. Learn more about this technique here.

March 1, 2016, 3:30 p.m.

The International Mentorship Program brings together students and mentors across Europe to develop the skills needed to succeed in a globalized world.

Nov. 6, 2015, 3:30 p.m.

Get tips on choosing the best lab for your graduate or postdoc work.

Oct. 2, 2018, 12:56 p.m.

Learn about a wide variety of potential solutions to reproducibility issues in the biological sciences including changes to scientific sharing, publishing practices, and experimental design.

May 18, 2018, 7:32 p.m.