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We narrowed to 628 results for: Dos

Showing: 361 - 380 of 628 results

  1. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...components can interfere with conjugates. Make sure to do your research before conjugating at the bench.   ...to distinct, non-overlapping epitopes so that they do not interfere with each other. The detection antibody...

  2. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...gradients, practice making the iodixanol layers before doing your first purification. This helped me get a feel...to learn more about different ways to titer AAV. Do you have any AAV production tips or tricks? Please...multiplicity of infections (MOIs), to determine the optimal dose of AAV for my cells of interest. Every batch of ...

  3. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...Gene X in the untreated cells—but can we be certain? Do we know whether the plasmid delivered into the cells...negative controls that produce a similar product, but do not target an endogenous gene in your experimental...

  4. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...of necessary data requirements and likely will not do so (calling it a “sisyphean task” given the endless...Firstly, we rely on many other labs to teach us how to do experiments and understand their data. Likewise, ...

  5. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...expect given the excitement surrounding anything to do with CRISPR, our most popular post in July (with ...

  7. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ... Michael Früh submission, which we have entitled "Do You See What I See?" I was bored this weekend while...

  9. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ...wild-type and engineered variants of Cas nucleases. In doing so, they also created a modular toolkit for Cas-...

  10. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...all the excitement and speculation, we thought we’d do a little digging and assemble a rough guide to the...

  12. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...being published on our own topic. Only on occasion do we then have time to read about research from other...otherwise never learn from the valuable research we are doing. Browse the site, learn from others, share ideas...

  13. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...sites, or you can utilise several online tools that do it for you. One such option is the CRISPR Design ... take roughly 3 months from the day you decide to do a gene modification experiment in mice to the day...

  14. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...editor. Cas13 enzymes are advantageous because they do not require a PAM, and RNA targeting is potentially...flanking sequence (PFS) adjacent to the target, but many do not, showing the flexibility of this system.  As ...

  16. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...tool that is easy to use with other plasmids.  How do you know if your plasmids are incompatible? Scientists...will be based on sequence data. However, if these do not provide clarity, a wet lab transformation experiment...

  17. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ... using site-directed mutagenesis of Spinach. In doing so, they identified the nucleotides that contribute...Just as vegetables come in a variety of colors, so do aptamers! Corn was recently described in 2017 (Song...

  18. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...guest blogger Derek Simon. The actual experiments you do will be determined by the topic you’re interested...Second, this may sound rudimentary, but before you do any type of behavior analysis, simply take a few ...

  19. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...yourself, you’re probably wondering: What does it do and how does it work? LentiCRISPR v2 purpose As its...

  20. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ...Education & Tools menu Further down What happens if you do scroll down? You’ll find Blugene, as well as information...
Showing: 361 - 380 of 628 results