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We narrowed to 622 results for: Dos

Showing: 361 - 380 of 622 results

  1. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...expression. To design a viral vector system that could do both, Addgene depositor Didier Trono and collaborators... lentiviral system (Figure 2). The HIV genes that do remain are very important for viral production: Gag...

  2. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...Gene X in the untreated cells—but can we be certain? Do we know whether the plasmid delivered into the cells...negative controls that produce a similar product, but do not target an endogenous gene in your experimental...

  3. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...of necessary data requirements and likely will not do so (calling it a “sisyphean task” given the endless...Firstly, we rely on many other labs to teach us how to do experiments and understand their data. Likewise, ...

  5. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ... Michael Früh submission, which we have entitled "Do You See What I See?" I was bored this weekend while...

  7. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...expect given the excitement surrounding anything to do with CRISPR, our most popular post in July (with ...

  8. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ...wild-type and engineered variants of Cas nucleases. In doing so, they also created a modular toolkit for Cas-...

  9. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...all the excitement and speculation, we thought we’d do a little digging and assemble a rough guide to the...

  11. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ... using site-directed mutagenesis of Spinach. In doing so, they identified the nucleotides that contribute...Just as vegetables come in a variety of colors, so do aptamers! Corn was recently described in 2017 (Song...

  12. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...editor. Cas13 enzymes are advantageous because they do not require a PAM, and RNA targeting is potentially...flanking sequence (PFS) adjacent to the target, but many do not, showing the flexibility of this system.  As ...

  14. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...tool that is easy to use with other plasmids.  How do you know if your plasmids are incompatible? Scientists...will be based on sequence data. However, if these do not provide clarity, a wet lab transformation experiment...

  15. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...being published on our own topic. Only on occasion do we then have time to read about research from other...otherwise never learn from the valuable research we are doing. Browse the site, learn from others, share ideas...

  16. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...sites, or you can utilise several online tools that do it for you. One such option is the CRISPR Design ... take roughly 3 months from the day you decide to do a gene modification experiment in mice to the day...

  17. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...guest blogger Derek Simon. The actual experiments you do will be determined by the topic you’re interested...Second, this may sound rudimentary, but before you do any type of behavior analysis, simply take a few ...

  18. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ...Education & Tools menu Further down What happens if you do scroll down? You’ll find Blugene, as well as information...

  19. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...yourself, you’re probably wondering: What does it do and how does it work? LentiCRISPR v2 purpose As its...
Showing: 361 - 380 of 622 results