962 results

Addgene remains steadfast in our mission to accelerate research and discovery by improving access to high-quality scientific materials and information. Our agility as a nonprofit resource can prove to be vital during this period of constrained funding.

April 23, 2025, 1:30 p.m.

This post covers loading controls and normalization of semi-quantitative western blots. We cover housekeeping proteins and total protein loading controls.

April 8, 2025, 1:15 p.m.

This post describes CRISPR-derived base editors that catalyze base transversions, such as purines like A or G to pyrimidines like T or C.

April 1, 2025, 1:15 p.m.

This post describes CRISPR-derived base editors to convert cytosine to adenine or guanine, called CGBEs or GBEs.

March 25, 2025, 1:15 p.m.

Learn about how CRISPR systems are classified. Addgene's guide to typing CRISPR.

March 18, 2025, 1:15 p.m.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

March 11, 2025, 1:15 p.m.

Addgene Executive Director Chonnettia Jones reflects on highlights of 2024 and shares exciting plans for 2025.

Feb. 25, 2025, 2:15 p.m.

An overview of cytosine and adenine base transition editors, which make precise changes in DNA bases without causing double-strand breaks.

Feb. 13, 2025, 2:15 p.m.

Learn about how Cascade-Cas3 can be used as a CRISPR tool to degrade large fragments of DNA.

Feb. 11, 2025, 2:15 p.m.

Changes in export control regulations have make rabies and VSV-G plasmids more accessible to researchers around the world.

Feb. 6, 2025, 2:15 p.m.

Learn about the advantages of using AAV as a delivery method for CRISPR screens.

Feb. 4, 2025, 2:15 p.m.

Addgene adds new ready-to-use viral vectors to our service every month. Read all about the latest additions here!

Jan. 28, 2025, noon

This post provides tips for designing a prime editing experiment, from pegRNA design, PE tool selection, and choice of DNA sequence to edit.

Jan. 23, 2025, 2:15 p.m.

Learn about the most common injection routes in mice, including their key differences and uses.

Jan. 22, 2025, 9:42 p.m.

Making the transition from bench to management can be daunting, but these tips can help!

Jan. 21, 2025, 2:15 p.m.

Adeno-associated virus (AAV) vector serotypes can help you target specific cell types through their tissue tropism.

Jan. 16, 2025, 2:15 p.m.

Addgene's AAV Packaged on Request option has officially launched! Learn more about how this expansion of our helpful viral vectors service can help your research.

Jan. 10, 2025, 1:01 p.m.

This Twelve Days of Antiboides, we're celebrating some cool new additions to our antibody repository and resources.

Jan. 6, 2025, 5:17 p.m.

Twenty years have gone by fast! Join us to share some highlights of Addgene and our community as we close out our 20th anniversary celebration.

Dec. 19, 2024, 2:15 p.m.

A round up of guest blog posts of 2024. Thank you to our guest contributors!

Nov. 26, 2024, 2:15 p.m.

Learn how Addgene has worked to increase accessibility to our materials and educational resources.

Nov. 21, 2024, 10:47 p.m.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Nov. 21, 2024, 1:30 p.m.

Addgene has a new animation showing the common ways antibodies are used to study proteins in the lab!

Nov. 19, 2024, 2:15 p.m.

Did you know CRISPR can be used to target non-coding RNAs? In this blog post, we explain the pros and cons of different approaches.

Nov. 14, 2024, 2:15 p.m.

Learn about the differences in virus production between insect cells and mammalian cells.

Nov. 5, 2024, 8:32 p.m.

Antibody data repositories and search engines can help you find the right antibody for your experiment. Check out a list of helpful links to get you started.

Oct. 31, 2024, 1:15 p.m.

Introducing the Antibodies 101 eBook, the latest addition to Addgene's helpful eBook collection!

Oct. 29, 2024, 1:15 p.m.

Congratulations to Addgene depositor David Baker, who was awarded the Nobel Prize in Chemistry last week alongside Demis Hassabis and John Jumper!

Oct. 17, 2024, 1:15 p.m.

Starting in January 2025, Addgene's Viral Vector service will include a Packaged on Request option.

Oct. 15, 2024, 1:15 p.m.

Addgene has updated our useful CRISPR guide to include PRIME editing, new enzymes, and more!

Oct. 10, 2024, 1:15 p.m.

Where did plasmids come from? Learn more about natural plasmids, the benefits they offer cells, and how scientists have adapted them into laboratory tools.

Oct. 8, 2024, 1:15 p.m.

Addgene adds new ready-to-use viral vectors to our service every month. Read all about the latest additions here!

Oct. 3, 2024, 1:15 p.m.

Sequence and ligation independent cloning (SLIC) lacks the strict requirements of many standard cloning techniques. Learn how to use SLIC in this post.

Sept. 26, 2024, 1:15 p.m.

Magnetoresponsive proteins have seemed like a dream... but Andrew York's lab has developed and deposited one, opening the possibility of magnetoresponsive tools!

Sept. 24, 2024, 1:15 p.m.

Addgene's excited to launch a new series of YouTube videos: Quick Tips!

Sept. 19, 2024, 3 p.m.

A dive into troubleshooting and optimization steps you can take to improve your western blot or identify and fix issues within the assay.

Sept. 17, 2024, 1:15 p.m.

Addgene's Antibody Data Hub can help you select the antibodies for your application.

Sept. 12, 2024, 1:15 p.m.

If you can't find a validated antibody for your flow experiment, this blog post can help you validate your antibodies yourself.

Sept. 10, 2024, 1:15 p.m.

There are a few different types of Fab fragments to consider when you need a smaller affinity reagent than antibodies. Learn about them here!

Sept. 5, 2024, 1:15 p.m.

Fragmid is a quick, easy way to design and create CRISPR plasmids. Read about the Doench lab's new modular cloning-based approach in this blog post!

Aug. 29, 2024, 1:15 p.m.