We narrowed to 72 results for: lentivirus vector

Learn about the many ways you can deliver reprogramming factors to generate induced pluripotent stem cells (IPSCs).

April 17, 2018, 1:37 p.m.

Get tips for producing, purifying, and tittering AAV, a tick but useful viral vector of choice for many people working to express genes in mammalian cells.

Oct. 23, 2018, 11:49 a.m.

The REPROcode collection includes 408 barcoded lentiviral vectors carrying immune-specific transcription factors, allowing transdifferentiation screens.

June 23, 2026, 1:15 p.m.

A review of protein expression mechanisms for on, off, and tunable, control of protein expression.

Feb. 16, 2023, 7:05 p.m.

Addgene Executive Director Joanne Kamen's discusses the new Viral Service, it's sponsors, and how it helps scientists accelerate their research.

March 23, 2017, 2:30 p.m.

Epigenetic modifications are an additional layer of control over gene expression. Targeted epigenetic modification is possible using CRISPR tools from Addgene!

June 24, 2020, 5:45 p.m.

Addgene reached its 15 year anniversary on January 8, 2019. In this blog post, find the top 15 requested plasmids since our founding. Many of these include lentiviral or CRISPR tools!

Jan. 8, 2019, 1:54 p.m.

Learn about some of the factors you should take into consideration when choosing the appropriate AAV for your next experiment.

June 13, 2017, 2:30 p.m.

X-CHIME, a series of CRISPR tools developed and deposited by Arlene Sharpe's lab, allows for germline knockouts in immune cells in mice.

Jan. 16, 2024, 6:13 p.m.

We recently attended the Society for Neuroscience conference in Washington DC. Check out some of what we learned about this exciting field.

Dec. 15, 2017, 2:07 p.m.

Find some tips and tricks for using CRISPR for gene editing.

March 7, 2017, 3:30 p.m.

Check out all of the plasmids featured in our hot plasmids articles from 2016.

Dec. 22, 2016, 3:03 p.m.

Learn how and why Adeno-associated virus (AAV) has enabled breakthroughs in retinal gene therapy.

July 31, 2018, 12:55 p.m.

Drive-and-proccess (DAP) arrays are a new tool from the Gao lab that allows for up to 31-loci CRISPR multiple base editing.

July 7, 2022, 1:15 p.m.

Find information on CRISPR mammalian expression systems and delivery methods. Use the table to quickly compare between the methods.

Sept. 24, 2015, 2:30 p.m.

This post provides an overview on how transcription terminates in prokaryotes and eukaryotes.

March 31, 2016, 2:30 p.m.

CellTag is a lentiviral based tagging strategy that combines scRNA-seq and lineage tracking at the single-cell level.

May 9, 2019, 1:15 p.m.

This month's Hot Plasmids articles features new plasmids, new antibodies, and a viral ticker tape system!

Nov. 14, 2023, 3:35 a.m.

Find plasmid tools for COVID-19 research expressing viral open reading frames, host factors, and tools for detecting SARS-CoV-2 and generating pseudoviruses.

July 12, 2021, 1:15 p.m.

An introduction to transposons including Sleeping Beauty, piggyBac, and Tol2 and how they can be used in transposon mutagenesis screens, gene transfer, and creating transgenic animals.

July 6, 2021, 1:28 p.m.

Learn how and why AAV vectors are being used in cell and gene therapies.

Nov. 7, 2017, 1:59 p.m.

Learn how to design a CRISPR screening experiment targeting enhancers.

Aug. 9, 2016, 2:30 p.m.

We describe the different modular cloning kits available for different applications and organisms to help you find the kit that's right for your experiment.

May 14, 2024, 1:15 p.m.

Learn how stem cell researchers use reprogramming and transdifferentiation techniques to convert cells from one type into another.

Nov. 6, 2018, 1:12 p.m.

Learn how AAVs can be used to trace neuronal connections.

Aug. 9, 2018, 1:04 p.m.

Learn how Addgene has worked to increase accessibility to our materials and educational resources.

Nov. 21, 2024, 10:47 p.m.

Genome-wide CRISPR/Cas9 screens are a high-throughput systematic approach for identifying genes involved in a biological process. Recently the Bassik Lab at Stanford developed a new genome-wide CRISPR knockout screen to analyze unanswered questions about CRISPR screen design by including safe targeting guides and short gRNAs.

Sept. 13, 2018, 1:55 p.m.

22 of the interesting and popular hot plasmid technologies of 2014 from Addgene's plasmid repository.

Jan. 6, 2015, 5:21 p.m.

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

June 28, 2018, 3:01 p.m.

Read on for a sampling of some of the exciting new plasmid technologies that have come through our doors in 2015.

Dec. 23, 2015, 3:30 p.m.

Learn about the pros and cons of biased and unbiased CRISPR genotyping methods.

Oct. 4, 2016, 2:30 p.m.

Learn about how ClonTracer, a lentiviral library for lineage tracing, was used to test if cancer cells have resistance to drugs before or after treatment.

Sept. 22, 2015, 2:30 p.m.