We narrowed to 118 results for: Cre

Researchers inactivate genes to investigate their function. This article describes techniques/plasmids used to knockout and knock-in specific genes in mammalian cells.

Dec. 1, 2016, 3:30 p.m.

Learn about the sources of spontaneous recombination and optimizing Cre-recombinase dependent AAV vector expression in vivo.

Nov. 2, 2021, 1:15 p.m.

Learn about the basic elements that make up the AAV transfer plasmid, including the ITRs, transgene, promoter, and other cis-regulatory elements.

Aug. 11, 2020, 1:15 p.m.

A toolkit of synthetic promoters for transgene expression in AAV can be used for targeted gene expression in retinal cell types in mice, primate, and human retinas.

March 12, 2020, 1:15 p.m.

iTOP is a protein-only transduction method for delivering proteins and RNA into a variety of cell types, with potential applications with CRISPR/Cas9.

June 23, 2015, 8:37 p.m.

Addgene adds new ready-to-use viral vectors to our service every month. Read all about the latest additions here!

Oct. 3, 2024, 1:15 p.m.

The CombiGEM technology allows a researcher to design and screen CRISPR gRNA libraries for effects on your biological phenomenon of choice.

April 12, 2016, 2:30 p.m.

Learn how you can use Green Listed to design gRNAs for custom CRISPR screens.

July 11, 2017, 2:30 p.m.

The yeast two hybrid (Y2H) assay is useful for probing interactions between proteins. Learn more about the Y2H assay and how you can use it in this post.

Oct. 22, 2015, 2:30 p.m.

Learn about recently available viral vectors and viral vectors coming soon from Addgene's viral service including calcium sensors, GABA sensors, voltage sensors, and control AAV.

March 26, 2019, 1:40 p.m.

Read about Gladys Kaba, the 3rd recipient of the Addgene/Seedinglabs Plasmid Grant, who will be using plasmids as positive controls in cervical cancer screens.

Nov. 8, 2016, 3:30 p.m.

Learn about some ways to study behavioral neuroscience with viral vectors using behavioral tests for locomotion, anxiety, and depression.

Sept. 17, 2019, 12:40 p.m.

Various lentiviral CRISPR-Cas9 libraries have been developed to perform genome-wide knockout screens, including the GeCKO library from Feng Zhang's lab.

Feb. 20, 2014, 4:57 p.m.

Check out how you can use the top lentivirus and AAV from 2016 in your own research.

Jan. 6, 2017, 3:56 p.m.

Learn about the advantages of using AAV as a delivery method for CRISPR screens.

Feb. 4, 2025, 2:15 p.m.

Interested in how Addgene creates our content, like blog posts and guides? We explain here!

Nov. 17, 2022, 2:15 p.m.

A general overview of how and when to use screens vs. selections and the ins and outs of each method.

Nov. 10, 2022, 2:15 p.m.

Learn about how to identify and develop creativity in the lab - a skill that's valuable in and out of the sciences!

Jan. 18, 2022, 2:15 p.m.

New to using CRISPR pooled libraries for your screen? Here, we cover how to choose a CRISPR library, and the steps and tips for a successful CRISPR screen.

Aug. 18, 2015, 2:30 p.m.

Blue-white screening is a well loved technique that allows scientists to quickly identify recombinant clones for further analysis.

June 4, 2015, 1:03 p.m.