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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 40 results for: RFP

Showing: 21 - 40 of 40 results

  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...) a RFP protein that binds to the 3’ UTR of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the...transcribed, it is bound by both the GFP and the RFP proteins in the nucleus. During the first round of...nucleus (thanks to a NLS on these proteins). The RFP proteins, however, remain bound to the mRNA since...Pre-constructed Entry vectors containing Cas9, EGFP, mCherry, iRFP, tdTomato, luciferase, LacZ, puromycin or CreERT2...vector expressing a separate reporter gene (EGFP, iRFP, IFP1.4, puromycin, neomycin or luciferase) to create...

  3. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ... by red fluorescent proteins such as tdTomato or RFP or blue fluorescent proteins like Cyan. The issue...

  4. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...LSSmOrange mRuby2 Red Mammalian Expresses mRuby2 (a RFP variant) commonly used with Clover pGWF1 Cyan &...

  5. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...-IRES-EGFP IRES Retroviral pMSCV-pBabeMCS-IRES-RFP IRES Retroviral pMSCV-IRES-YFP II IRES Retroviral...

  6. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...Enhanced miRFPs are monomeric and bright Recent series of enhanced monomeric NIR FPs named miRFPs (monomeric...Therefore, we recommend using miRFPs and their enhanced versions named emiRFPs in your applications.  There...set of spectrally distinct (e)miRFPs available. The numbers in (e)miRFP names correspond to the maxima...multicolor NIR imaging.  Small miRFPnano A separate class of NIR FPs, named miRFPnanos, were derived from cyanobacteriochrome...advantages are their small size (17 kDa for miRFPnanos, 35 kDa for miRFPs, and 27 kDa for EGFP) and a possibility... of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular and molecular brightness.... FRET biosensors were developed based on miRFP760-miRFP720 FRET pair, including Rac1 biosensor (Shcherbakova...

  7. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ...phytochome-derived proteins, iRFP670, iRFP682, iRFP702, iRFP713 and iRFP720, is that they absorb and emit...near-infrared fluorescent proteins (FPs), called PAiRFP1 and PAiRFP2, to the in vivo imaging toolbox, which increase...application of near-infrared fluorescent proteins, iRFP670 and iRFP720, as photoacoustic contrast agents for two-color...that near-infrared range, and that’s exactly what iRFPs achieved. Those proteins allowed a signal-to-background...

  9. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...change that placesTagRFP-T and Dronpa in close proximity. By monitoring changes in TagRFP-T fluorescence...FLINC)-based biosensors measure fluctuations of TagRFP-T fluorescence intensity due to interactions with...membrane in living cells using a construct in which TagRFP-T and Dronpa flanked a PKA substrate fused to the...

  11. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ... containing several types of fluorescent (EGFP, tagRFP, mCherry) and nonfluorescent (Halo, SNAP) tags,...NanoLuc), fluorescent proteins (TagGFP2, TagBFP, TagRFP, and T2A-TurboGFP-PEST), and small epitope tags...

  12. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...can fold in <10min at 37°C, mCherry takes ~15min, TagRFP ~100min and DsRed ~10hours. Temperature: FPs maturation...is set as 1. Some proteins are very dim (e.g. TagRFP657, which has a brightness of 0.1) and this should...

  13. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...small monomeric NIR fluorescent proteins (miRFP670nano and miRFP720) and the Ca2+-sensing domain from Twitch...

  14. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...Serotype Category Depositor  Notes pAAV-CAG-emiRFP670 AAV1 Control Kiryl Piatkevich New viral tool ...

  15. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...anti-FLAG frankebodies includes mEGFP, mRuby2, iRFP713, SNAP-tag, and HaloTag fusions, while the anti-...

  18. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...inducible promoter. The gRNA piggyBac plasmids include a mRFP reporter. With this system, they could quantify ...
Showing: 21 - 40 of 40 results