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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 31 results for: biotin

Showing: 21 - 31 of 31 results

  1. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...captures the streptavidin portion of the probe via biotin. The fluorescent portion of the probe that is cut...target sequence is amplified by PCR/RPA using biotinylated primers. These are then immobilized with streptavidin...that contain streptavidin which can bind to the biotinylated targets. They also tried to confirm target binding...

  2. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...counterpart label nearby proteins with biotin. The biotinylated proteins can then be isolated and identified...

  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...streptavidin-binding peptide (SBP). The addition of biotin disrupts this interaction and thus triggers a synchronous...

  6. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...Generation Toolbox! Starting with libraries and a biotinylated (for purification purposes) protein target of...process, displayed sybodies that bind to the biotinylated target protein can be recovered for the subsequent...plate to capture Myc-tagged sybodies. Then, the biotinylated target protein is added and after a few washes...

  7. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...bi-directionality. For predominately anterograde tracing, biotinylated dextran amines (Veenman et al., 1992) or fluorescent...Veenman, C.L., Reiner, A., and Honig, M.G. (1992). Biotinylated dextran as an anterograde tracer for single-...

  9. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...Combo Consists of a bacterially derived in-vivo biotinylation signaling peptide (Bio), flanked by hexahistidine...

  10. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...streptavidin-coated magnetic beads through an assembly of biotinylated SpyTag-SpyCatcher linkers and spacer proteins...

  11. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...need more signal, another strategy is to use a biotinylated secondary antibody then label that with fluorescently-conjugated...
Showing: 21 - 31 of 31 results