CRISPR Guide
Type
Guide
...by the Jian Xu lab , utilizes biotin tagging of dCas9 by fusing a biotin acceptor site to dCas9 and co-expressing...system can use common tags, like 3xFLAG-tag, PA, and biotin tags, or an anti-Cas9 antibody. The CAPTURE system...co-expressing BirA biotin ligase. Whatever the chosen epitope tag, the locus is subsequently isolated ...