We narrowed to 236 results for: cloning

Learn how to use and apply NEBuilder HiFi DNA Assembly Technology from New England Biolabs for quick and accurate plasmid cloning.

Sept. 6, 2017, 1:01 p.m.

Colony PCR is a useful technique for quickly screening for successful constructs after a plasmid cloning experiment.

May 12, 2016, 2:30 p.m.

Learn more about the common E. coli strains used in the lab.

Nov. 7, 2014, 2:56 p.m.

Using plasmids for transformation, cloning, or transfection? Different uses mean you'll need different purities of DNA for your applications.

June 16, 2020, 1:25 p.m.

Blue-white screening is a well loved technique that allows scientists to quickly identify recombinant clones for further analysis.

June 4, 2015, 1:03 p.m.

Fragmid is a quick, easy way to design and create CRISPR plasmids. Read about the Doench lab's new modular cloning-based approach in this blog post!

Aug. 29, 2024, 1:15 p.m.

A brief summary of the history of plasmids and their use in cloning and molecular biology.

Oct. 29, 2015, 2:30 p.m.

A list of free online molecular biology tools for plasmid mapping, DNA and protein sequence analysis, primer design and more.

Sept. 12, 2023, 1:15 p.m.

GreenGate is a cloning system for rapidly assembling plant transformation constructs and is based on the Golden Gate method.

Jan. 23, 2014, 3:23 p.m.

New to plasmids? Learn the basics of plasmids in this blog post and find out about the parts of a plasmid, how they are made, and how scientists can use them in the lab.

April 2, 2020, 6:17 p.m.

22 of the interesting and popular hot plasmid technologies of 2014 from Addgene's plasmid repository.

Jan. 6, 2015, 5:21 p.m.

Interview with Nicola Patron about her work in plant synthetic biology and her Golden Gate MoClo plasmid kit.

June 12, 2014, 3:30 p.m.

Find out what were Addgene's most popular blog posts in 2020!

Dec. 29, 2020, 2:15 p.m.

Learn about different strategies used to generate plasmids for multiplex gRNA expression and how to use them in various organisms.

Sept. 10, 2020, 11:45 a.m.

An exploration of how the Trimmer lab developed their Addgene-deposited collection of recombinant antibodies.

April 5, 2022, 1:20 p.m.

Lambda Red mediated recombineering can be used to generate point mutations in plasmids and bacterial genomes. Learn how to use Lambda Red in this post.

Dec. 15, 2016, 3:57 p.m.

REPLACR-mutagenesis is a new in vivo method for mutating your plasmid constructs. Learn how REPLACR stacks up against other mutagenesis techniques.

Feb. 10, 2016, 3:30 p.m.

This blog series showcases some of the new CRISPR tools we have at Addgene. Read about CRISPR-Cas12b for human genome editing, plant base editors, string assembly gRNA cloning, lineage tracing tools, and more.

March 21, 2019, 12:47 p.m.

Choose the best empty backbone for your experiment from some of the most popular in Addgene's library.

Aug. 19, 2014, 3:39 p.m.

Learn about the importance of antibiotic resistance genes in plasmids. How they work, how to use them, and some great tips.

Jan. 30, 2014, 3:29 p.m.