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We narrowed to 675 results for: eng

Showing: 21 - 40 of 675 results

  1. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...enzymes are the latest tools in the transcriptome engineering toolbox. How were Cas13d enzymes discovered?...property is size - Cas13d enzymes have a median length of ~930aa - making them 17-26% smaller than other... tell how large the genome and transcriptome engineering toolbox will be. It is, however, certain that...References Konermann, Silvana, et al. “Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors...

  2. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    Published
    July 28, 2017, 1:30 p.m.
    ...provide a brief introduction as to how you can engineer new transgenic flies to study your favorite gene...about Using Cre in Drosophila Read Other Genome Engineering Posts Use FLEx Vectors for Site-Specific Recombination... genome engineering...

  3. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...method that can be used for cloning or genome engineering and is based on homologous recombination. It ...lambda red bacteriophage and its use as a genetic engineering tool is frequently called recombineering - short...for homologous recombination-mediated genetic engineering.  It can be used to make an assortment of modifications...efficient recombination system for chromosome engineering in Escherichia coli. PNAS 2000 97 (11) 5978-5983...Homologous Recombination-Based Method of Genetic Engineering. Nature protocols. 2009;4(2):206-223. doi:10.1038...and Court, D. L. 2014. Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination. Current...Mammalian Cells CRISPR Methods for Bacterial Genome Engineering Resources on Addgene.org Browse our Microbiology...

  4. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...by Benoit Giquel. CRISPR has taken the genome engineering world by storm owing to its ease of use and utility...Waltz, 2016), the potential of CRISPR for genome engineering in plants is also being realized. There are a...plants with the phenotype of interest. Genome engineering, on the other hand, allows for targeted modification...phenotype. In fact, CRISPR has already been used to engineer the genome of many plant species, including commonly...will present a general overview of plant genome engineering using CRISPR, highlight the specific modifications...plants, and outline the various plant genome engineering tools that are available to academic researchers... Addgene. CRISPR components for plant genome engineering CRISPR can be used to knockout, activate or repress...

  5. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    Published
    May 22, 2018, 1:38 p.m.
    ...students (1, 2), but educators face significant challenges in designing suitable projects. In the O'Connor...Advisors on Science and Technology [PCAST] (2012). Engage to Excel: Producing One Million Additional College...Graduates with Degrees in Science, Technology, Engineering and Mathematics, Washington, DC: US Government...

  6. Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew

    Type
    Blog Post
    Published
    Feb. 6, 2018, 2 p.m.
    ...career path and why he chose to go into genome engineering. Chapter 1: Early research on CRISPR and what... 6: Projects in Wei Leong Chew's lab. Genome engineering, gene delivery methods, AAVs, and more. Chapter...and gene therapy.   Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional... genome engineering...

  7. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...bacterial CRISPR engineering One common way of accomplishing bacterial genome engineering is done with recombineering...the genus Streptomyces, yet genome engineering remains challenging for most organisms in the genus. Work...discovered in bacteria, most CRISPR-based genome engineering has taken place in other organisms. In many bacteria...quirks change the way CRISPR-mediated genome engineering functions in bacteria, but have no fear - plasmids...recombineering-based MAGE (Multiplex Automated Genome Engineering) technology (Ronda et al., 2016). pMA7CR_2.0 ...E. coli and T. citrea scarless editing plasmids Sheng Yang’s lab describes a two-plasmid system that combines...create a system for scarless, iterative genome engineering (Jiang et al., 2015). pCas contains Cas9 and ...

  8. Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

    Type
    Blog Post
    Published
    Nov. 12, 2014, 3:54 p.m.
    ...multiplex genome engineering. Image from Sakuma at al. (2015). Multiplex genome engineering demonstrated...system for CRISPR/Cas9-mediated multiplex genome engineering is now available thanks to researchers at Japan...making an all-in-one vector for multiplex genome engineering.” Now, they’ve filled that gap. As Sakuma and...efficient viral vector-mediated multiplex genome engineering. The system now provides an efficient targeting...Sakuma’s protocol. Reference: Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector... multiplex genome engineering...

  9. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. doi: 10.1186/1754-1611-8-7. PubMed...has opened up a world of possibilities in the engineering of complex gene networks. A comparable system...assemble designer PUF domains for RNA specificity engineering. “The RNA binding domain is interesting because...report published in The Journal of Biological Engineering in March is fused to a translational repressor...

  11. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...within tumours via focused ultrasound. Nat Biomed Eng. 2021 Nov;5(11):1336-1347. doi: 10.1038/s41551-021...antibody?  Similarities:  Generated against full length GFP and converted to recombinant form by the NeuroMab...these processes. The Liu and Petsko labs recently engineered hyperfolder YFP (hfYFP), a FP well-suited for...

  12. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ... Glanville J, Wolf P, Frenzel A, Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018)...

  13. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    Published
    May 4, 2021, 1:15 p.m.
    ... Glanville J, Wolf P, Frenzel A, Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018)...empower the scientific community with the tools to engineer them. Given the ease with which scientists can... Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched...Monoclonal Antibodies for Clinical Use. In: Antibody Engineering. InTech Additional resources on the Addgene blog...

  15. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...their applications in neuroscience. Curr Opin Biomed Eng. 2019. 12:111-117. doi: 10.1016/j.cobme.2019.10.010... as bright as the other GEVI systems that use engineered FPs. Attempts have been made to optimize their...their fluorescence, which ultimately led to the engineering of the second type of these GEVIs: a FP-fused... voltage sensor. FP-rhodopsin GEVIs have been engineered to have kinetics comparable to its one component... transiently and for genomic integration. Lab-engineered viruses will package your genetic cargo in lieu...

  16. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...of mice via adeno-associated viruses. Nat Biomed Eng 4:97–110 . https://doi.org/10.1038/s41551-019-0501...gRNA together (~4.2 kb) into an AAV vector is challenging due to its packaging capacity of AAV (~4.7 kb... little room for additional regulatory elements. Feng Zhang's group previously packaged Cas9 and multiple...enough to package into AAV. At 984 amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is the...ADAR2DD(delta984-1090) are approximately 4.1 kb in length, allowing them to be packaged in AAV.   Split ...transgenes might seem daunting, it’s actually a challenge that the field has overcome with split AAVs. In...When a cell is transduced by both AAVs, the full length gene and/or protein is reconstituted. One way to...

  17. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ...enabling technologies in synthetic biology. J. Biol. Eng. 2013; 7, 13. PubMed PMID: 23663447. PubMed Central...fragments of the chromosome, which was technically challenging. Other homology based technologies We've described...Central PMCID: PMC3874208 Other Resources: New England Biolabs Gibson Assembly Overview Additional Resources...Protocols Gibson Assembly® is licensed to New England Biolabs by Synthetic Genomics, Inc. ...

  18. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...mechanobiology: seeing is believing." Annu. Rev. Biomed. Eng. 10 (2008): 1-38. PubMed PMID:18647110. Additional...spectral properties upon exposure to a specific wavelength of light. PA-FPs can be activated from low fluorescent...brighter fluorescent state by exposure to a specific wavelength of light. The switch or photoconversion usually...multiple times by activating with two distinct wavelengths of light (see Table 2). The best known reversible...photoactivation) (6). Dronpa is photoactivated by blue wavelengths of light, causing emission of green fluorescence...another group of reversible PA-FPs. These were engineered to be the first reversible, monomeric red fluorescent...dynamics (fission, fusion, etc). For example, by engineering PA-GFP tagged to a mitochondrial matrix protein...

  19. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...2014. PubMed PMID: 24151867 Mikkelson, et al. Metab Eng. 2012. PubMed PMID: 2232647   SapTrap, a toolkit...genome engineering across multiple species Recent discoveries in the fields of genome engineering and synthetic...pCAGGS-TAL-NC2 Charles Gersbach pcDNA3.1-GoldenGate, pcDNA3.1-GoldenGate-VP64 Maria-Elena Torres-Padilla pTALYM3...fold a protein because of its length. Mark Howarth’s lab recently engineered a new peptide/protein pair,...as not only a protein engineering tool, but also as a way to characterize/engineer promoters, enhancers... developed for the study of enhancer function, strength, and binding (e.g. DHS-seq, FAIRE-seq, and CHIP-seq...quantification of enhancer activity have remained challenging due to the variable availability of enhancer ...

  20. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...ways of delivering healthcare. Clients typically engage consultants as short-term experts to work on “cases...Cases present specific operational or commercial challenges or questions that clients face imminently. Consulting...on analyzing the situation that presented the challenge at hand and help resolve it through well-informed...specialize in business strategy tend to be mostly engaged by pharma and biotech clients. In this realm, there... there are a few different kinds of challenges that consultants get called upon to tackle, depending on...to collaborate whenever it faces an important challenge. The types of projects mentioned above are just...rewarding parts of the job is being confronted with challenges that are crucial for the success of a drug’s ...
Showing: 21 - 40 of 675 results