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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 45 results for: flag

Showing: 21 - 40 of 45 results

  2. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...et al. "Transgenic mouse lines expressing the 3x FLAG‐dC as9 protein for enCh IP analysis." Genes to cells...cells by expressing dCas9 with an N-terminal 3xFLAG tag (3xFLAG-dCas9) and a gRNA targeting their genomic... In addition to 3xFLAG and biotin tags, use of tag systems including Protein A, 1xFLAG, 2xAM, and HA have...Immunoprecipitation, using an antibody targeting the 3xFLAG tag, allowed these researchers to isolate dCas9...chromatin or DNA fragment (Fujita et al., 2016). 3xFLAG-tag fused to dCas9, along with a biotinylated gRNA...

  3. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...(YFP, GFP, CFP, RFP, etc.) or affinity tags (HA, FLAG). There are also a variety of plant selection markers...

  4. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...identity and plasmid length. Any mismatch or indel flags the sequence for manual review by one of our QC ...

  7. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...expression measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate...

  8. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...generation lentiviral transfer vector. Each gene has been FLAG-tagged for convenient experimental use. The plasmids...summarized in the table below:   ID Cv (Capsid) FLAG Tag Terminus 79629 (N) and 79628 (C) Cv (capsid...

  9. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...been deposited with Addgene and include MYC- and FLAG-tagged TZAP constructs (wild-type and truncation...

  10. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...there are many types of epitope tags (eg. HA, MYC, Flag), they can be deleterious and cause aberrant protein...the genome. In this work, they use AAVS1_Puro_PGK1_3xFLAG_Twin_Strep and nuclease driven recombination ...

  11. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...intron between a fluorescent protein (FP) and a 3xFLAG tag (see construct schematic below.) After self-excision...synthetic intron, and only the fluorescent protein and 3xFLAG-tag sequences are inserted into the genome. ...repair template plasmid to create fluorescent and 3xFLAG-tag fusion proteins. The repair template and Cas9...

  12. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...by Paix et al. Although I was able to knock-in a 2xFLAG epitope into my gene of interest, I encountered...digests of PCR products used to identify a single 2xFLAG knock-in by direct screening. The center images...mutation significantly enriches for knock-in of 2x and 3xFLAG epitopes into other, non-linked loci; pha-1(e2123...

  13. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...the US and Canada convene at ComSciCon's annual flagship workshop.  Our 2018 workshop took place in Boston...students, and we pay for travel expenses to our flagship workshop. In our view, the best possible outcome...

  14. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...reach zooxanthellae, the coral’s symbiotic dinoflagellates that are photosynthetic (Salih et al., 2000... chromoproteins could be used for mimicry and camouflage. An anemone’s red colored tentacles resemble ...

  17. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ... or PreScission), epitope tag (12xHis, 2xStrep, 3xFlag, Protein A, or V5), and selection marker (KanMX...

  18. New Viral Vectors - Winter 2025

    Type
    Blog Post
    Published
    Jan. 28, 2025, noon
    ... AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) AAV-PHPeB ...
Showing: 21 - 40 of 45 results