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We narrowed to 74 results for: multi

Showing: 21 - 40 of 74 results
  1. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...HRP-RANbodies are, however, compatible with most multi-labeling immunohistochemical protocols where either.... Chicken IgY domains are especially useful for multi-color staining of tissue because they have limited...primary/ labeled secondary antibody. Compatible with multi-labeling protocols when other antigens are detected...
  2. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...indels at a single locus, usually the target site. Multi-locus asseEENdb database ssment would be required...between tools. Examples: “single-target design”, “multi-target design”, “off-target aware”, “high mismatch..., “empirical design”, “single-PAM design”, and “multi-PAM design”. Trends: By the end of 2014, tools began...plasmid design. Examples:  “multiplex design”, “multi-method design”, and “single-method design”. Trends...
  3. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ... coli, SLIC is a cheap, standardized, and rapid multi-part DNA assembly method. Read on to learn how to...Gibson assembly. SLIC is a standardized method for multi-fragment DNA assembly, and its low cost makes it...
  4. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...Specifically, these types of CRISPR systems work using a multi-Cas complex called Cascade (CRISPR-associated complex...fewer off-target alterations, likely due to the multi-Cas mechanism. These benefits of Cas3 do come at...
  5. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...I-F INTEGRATE using a single plasmid containing a multi-spacer CRISPR array. Workflow and technical ...multiplexed gene knockouts (Vo et al., 2020), to multi-copy genomic integration of metabolic cassettes ...
  6. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...Spaghetti Monster probes at Addgene Uses of smFPs 1. Multi-channel connectomic tracers Fluorescent proteins...antibodies. GFP emits light in only a single channel. In multi-color imaging experiments, the second color is usually...
  7. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...species and/or different IgG subclasses of 1° for multi-color staining Can be expensive Nanobodies   ...before application to the sample. Staining with this multi-color staining workflow yields similar localization...
  8. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ... to extreme sequence divergence that requires a multi-pronged analysis to sort effectively. The current...Class 1 and Class 2. Class 1 CRISPR systems use multi-Cas effectors, referred to as Cascade (CRISPR-associated...popular in the lab. This can be attributed to the multi-Cas effector, Cascade. Developing tools using a ...
  9. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...energize you. For example, let's say you’re doing a multi-day experiment in the lab. You have two ways to ...recently started using Trello to manage larger, multi-person projects. Day-to-day, flexible to-do lists...
  10. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ... fluorophore tagging vectors for your single or multi-color imaging experiments. Interested in epitope...
Showing: 21 - 40 of 74 results