We narrowed to 128 results for: pcr

Learn how to verify indels, deletions, and homology directed repair (HDR) events in your CRISPR modified cell lines.

Nov. 3, 2022, 12:15 p.m.

Learn about antibody engineering, including what it is and why we like it. Deep dive into antibody directed evolution techniques, and learn about Addgene's partnership with the Institute for Protein Innovation (IPI).

Aug. 5, 2025, 1:15 p.m.

In this troubleshooting guide, find step-by-step tips and tricks for troubleshooting your cloning experiment.

Sept. 24, 2019, 12:55 p.m.

Ever wondered how Addgene makes our viral preps? We explain it all here!

Oct. 24, 2023, 1:15 p.m.

Find some tips and tricks for using CRISPR for gene editing.

March 7, 2017, 3:30 p.m.

Learn how to use and apply NEBuilder HiFi DNA Assembly Technology from New England Biolabs for quick and accurate plasmid cloning.

Sept. 6, 2017, 1:01 p.m.

With the limitations of qRT-PCR based COVID-19 testing, scientists develop CRISPR-based diagnostic tools for COVID-19.

May 5, 2020, 1:15 p.m.

Learn how various lab techniques have evolved but still remain relevant in many research arenas today.

June 21, 2016, 2:30 p.m.

Gibson Assembly is a popular cloning alternative to traditional restriction/ligation methods. Learn more about this technique here.

March 1, 2016, 3:30 p.m.

Blog editor Tyler Ford runs down some of the best posts from August 2016. NgAgo, site directed mutagenesis, and much more!

Sept. 2, 2016, 1:32 p.m.

An exploration of how the Trimmer lab developed their Addgene-deposited collection of recombinant antibodies.

April 5, 2022, 1:20 p.m.

Find three ways to purify genomic DNA, plasmid DNA, or PCR products without a commercial kit. Also, find a method for regenerating silica columns for reuse.

Feb. 11, 2020, 2:15 p.m.

Scientists are not limited to restriction enzyme cloning. Gateway cloning is a rapid and efficient way to move DNA into multiple vector systems.

Jan. 12, 2017, 3:30 p.m.

Read about Gladys Kaba, the 3rd recipient of the Addgene/Seedinglabs Plasmid Grant, who will be using plasmids as positive controls in cervical cancer screens.

Nov. 8, 2016, 3:30 p.m.

SapTrap improves CRISPR genomic tagging in C. elegans by offering scientists an easy one tube assembly reaction to generate high-throughput targeting vectors.

Oct. 12, 2017, 2:26 p.m.

Learn what a high fidelity DNA ligase is and when you might need one.

March 6, 2018, 1:49 p.m.

How much time does it take to clone a plasmid and how much does it cost? We do some back of the envelope math to try and figure it out!

March 23, 2023, 1:15 p.m.

Learn how DNA methylation may affect the activity of certain restriction endonucleases.

June 30, 2016, 2:30 p.m.

Learn how to use recombinase-based state machines from the Lu lab to monitor cellular events and regulate gene expression.

July 28, 2016, 2:30 p.m.

Hear from the authors about the generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9.

Feb. 18, 2015, 3:09 p.m.