We narrowed to 8 results for: pcr

...add restriction sites to any piece of DNA during PCR amplification, allowing for it to then be digested...naturally leaves a single A overhang on the 3' end of PCR products. The complementary T comes from a pre-cut...an incredibly quick and easy way to clone a fresh PCR product into a plasmid. The major disadvantage of...are also available, allowing for rapid cloning of PCR products into donor plasmids without the need for...used, so if properly designed, any insert fragment (PCR product or synthesized oligo) with appropriate overhangs... add. Complementary overhangs are built into the PCR primers for the insert, based on the destination ...cloning by restriction digest Plasmid cloning by PCR DNA ligation Gibson assembly Ligation independent...

...methods like sequencing or digital PCR , RNA-based methods like qPCR , and/or protein-based methods like...Very inefficient Can't distinguish specific edits PCR Knockouts Large insertions/deletions Some prime and...provide specific sequence information alone Digital PCR Knockouts Insertions/deletions Prime and base edits...RNA-sequencing, Perturb-seq, deep sequencing, etc. qPCR Knockouts Activation/interference Needs good primer...

...surviving cells are collected, their plasmids are PCR-amplified and sequenced using NGS to identify their...

...single-stranded DNA sequence used as an initiation point for PCR amplification or DNA sequencing of the plasmid. Primers...

... is necessary for Cas enzymes to bind target DNA PCR P olymerase C hain R eaction; Used to amplify a specific...

...G-protein coupled receptors (GPCRs) . The largest class of cell surface receptors, GPCRs are seven-pass transmembrane...activates G-proteins to modulate downstream signaling. GPCRs have been shown to be involved in a wide variety...inflammation and neurotransmission. This, and the fact that GPCR ligands can have high potency, which is potentially...development into chemogenetic tools. Studies of GPCRs led to development of mutated receptors that while...that couples to β-arrestin to activate noncanonical GPCR signaling independent of G proteins. This DREADD... manipulate neuronal activity indirectly through GPCR signaling, another class of chemogenetic receptors...

...Yeast GPCR-sensor Toolkit Yeast Expression Tom Ellis Plasmids for constructing highly-tunable GPCR-based...

...Ultracentrifugation AAV Titration by ddPCR AAV Titration by qPCR Using SYBR Green Technology References...