Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 7 results for: pcr

Showing: 1 - 7 of 7 results

  1. Cloning

    Type
    Guide
    ...add restriction sites to any piece of DNA during PCR amplification, allowing for it to then be digested...a single adenosine (A) overhang on the 3' end of PCR products. The complementary thymine (T) comes from...an incredibly quick and easy way to clone a fresh PCR product into a plasmid. The major disadvantage of...are also available, allowing for rapid cloning of PCR products into donor plasmids without the need for...used, so if properly designed, any insert fragment (PCR product or synthesized oligo) with appropriate overhangs...add). Complementary overhangs are built into the PCR primers for the insert, based on the destination ...

  2. Plan Your Experiment

    Type
    Guide
    ...methods like sequencing or digital PCR , RNA-based methods like qPCR , and/or protein-based methods like...Very inefficient Can't distinguish specific edits PCR Knockouts Large insertions/deletions Some prime and...provide specific sequence information alone Digital PCR Knockouts Insertions/deletions Prime and base edits...RNA-sequencing, Perturb-seq, deep sequencing, etc. qPCR Knockouts Activation/interference Needs good primer...

  3. Guide to Using Pooled Libraries

    Type
    Guide
    ...surviving cells are collected, their plasmids are PCR-amplified and sequenced using NGS to identify their...

  4. Molecular Biology Reference

    Type
    Guide
    ...single-stranded DNA sequence used as an initiation point for PCR amplification or DNA sequencing of the plasmid. Primers...

  5. CRISPR Guide

    Type
    Guide
    ... is necessary for Cas enzymes to bind target DNA PCR P olymerase C hain R eaction; Used to amplify a specific...

  6. Chemogenetics Guide

    Type
    Guide
    ...coupled receptors, or GPCRs. The largest class of cell surface receptors, GPCRs are seven-pass transmembrane...receptors? Read the GPCRs: How Do They Work and How Do We Study Them? blog! Studies of GPCRs led to development...activates G-proteins to modulate downstream signaling. GPCRs have been shown to be involved in a wide variety...inflammation and neurotransmission. This, and the fact that GPCR ligands can have high potency, which is potentially...that couples to β-arrestin to activate noncanonical GPCR signaling independent of G proteins. This DREADD... manipulate neuronal activity indirectly through GPCR signaling, another class of chemogenetic receptors...
Showing: 1 - 7 of 7 results