We narrowed to 128 results for: pcr

PCR can be used to append the appropriate restriction sites to your insert and facilitate downstream cloning. Read on for tips on this process.

March 29, 2016, 2:30 p.m.

Colony PCR is a useful technique for quickly screening for successful constructs after a plasmid cloning experiment.

May 12, 2016, 2:30 p.m.

Use site directed mutagenesis to insert small mutations into your plasmid of interest and follow these tips for a smooth process with easy validation.

Aug. 2, 2016, 2:30 p.m.

Check out how you can use digital PCR to validate your CRISPR genome edits.

March 30, 2017, 2:30 p.m.

Find tips and tricks for using digital droplet PCR to quantify your AAV titers.

Jan. 16, 2020, 2:18 p.m.

an overview of qPCR and ddPCR copy number variant assays.

April 26, 2022, 1:15 p.m.

A deep dive into qPCR with a focus on analysis, chemistries, and bench tips.

Sept. 29, 2022, 1:15 p.m.

The pCri plasmids available through Addgene allow researchers to multiplex the protein purification process saving time and frustration.

June 19, 2015, 3:08 p.m.

An open-source biosensor platform, TRUPATH, measures the association of G protein signal transducers using bioluminescence resonance energy transfer.

Dec. 14, 2020, 2:15 p.m.

GPCRs are receptors that respond to stimuli and elicit cellular responses. Learn about the parts of GPCRs, how they work, and tools to study them.

Feb. 11, 2021, 2:15 p.m.

The basics of PCR covering end point PCR, qPCR, ddPCR, and multiplex PCR and how each method is used.

Aug. 10, 2021, 1:15 p.m.

FastCloning is a fast and easy technique that can replace restriction cloning. Learn about this great plasmid cloning technique here.

Nov. 1, 2016, 2:30 p.m.

TOPO cloning is a restriction enzyme and ligase free cloning method. Learn how TOPO works in this post.

Oct. 27, 2016, 2:30 p.m.

In Vivo Assembly (IVA) cloning uses the bacterial recombination pathway to allow any cloning procedure to be performed with a simple two-step, 2 hr protocol prior to transformation. This post covers the basics of the IVA cloning procedure, primer design, and tips & tricks for successful IVA cloning.

Oct. 18, 2018, 12:37 p.m.

REPLACR-mutagenesis is a new in vivo method for mutating your plasmid constructs. Learn how REPLACR stacks up against other mutagenesis techniques.

Feb. 10, 2016, 3:30 p.m.

Learn how we titer AAV for our viral service at Addgene and find tips for improving your AAV tittering methods.

Nov. 15, 2017, 3 p.m.

One-pot saturation mutagenesis can quickly generate libraries of mutant plasmids for phenotypic screening. Learn more from SciComm intern Beth Kenkel.

Feb. 22, 2017, 3:30 p.m.

There are many plasmid tools available in molecular biology so how do you chose the right cloning method for you? Here, consider speed, cost, difficulty, efficiency, and the number of inserts when designing your cloning strategy.

Aug. 21, 2018, 12:31 p.m.

Here, we cover four different plasmid screening strategies including blue-white screening, colony PCR, restriction digest analysis, and Sanger sequencing.

Sept. 15, 2020, 1:15 p.m.

Sequence and ligation independent cloning (SLIC) lacks the strict requirements of many standard cloning techniques. Learn how to use SLIC in this post.

Sept. 26, 2024, 1:15 p.m.