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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 33 results for: pcr

Showing: 1 - 20 of 33 results

  1. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...to perform PCR in step 6.3 . Assemble a 20 μl PCR with the following components: 10 μl 2x PCR mix, 0.5 ...each well) to a 96-well PCR plate using a multichannel pipette. Centrifuge PCR plate at 400 x g for 5 ... in prostate tumors by quantitative real-time PCR (qPCR). Gordanpour A, Nam RK, Sugar L, Bacopulos...biallelic modification, ease of rapid identification by PCR, predictability of loss-of-function, and utility ... genomic DNA (gDNA) for the intended deletion by PCR (see steps 6.1 and 7.1 ). The bulk cells have sufficiently...sgRNA-B (see step 5 ). The purpose of the following PCR is to validate primers and verify the presence of...Use the primers designed in step 2 above. Conduct PCR for “non-deletion band” and “deletion band” in separate...

  2. Viral Production

    Type
    Collection
    ... AAV particles are titered by droplet digital PCR (ddPCR) using primers and probes targeting the ITR elements...April 2016) also performed by droplet digital PCR (ddPCR). Quality Control Addgene ensures high quality...are titered using a lentiviral droplet digital PCR (ddPCR) titration protocol (modified from Wang et al...confirmed to have the correct integrated provirus using PCR. This assay involves transducing cells with serial...obtained using ddPCR are generally 2–3 fold higher than those achieved using the standard qPCR method. Titering...

  4. CRISPR Plasmids - Tagging

    Type
    Collection
    ... technique for use in Drosophila cells that uses PCR to generate both an expression cassette for the Cas9.... The plasmids in the following articles provide PCR templates for amplification of the tag (eg GFP, Flag...expresses Cas9, PITCh-gRNA, & FBL-specific gRNA pCRIS-PITChv2-FBL - PITCh donor vector for EGFP-2A-PuroR...

  5. Zhang Lab CRISPR Page

    Type
    Collection
    ... be co-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran et al., Nature Protocols...codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature Protocols 2013). Individual...

  6. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...need Cas9 plasmid Joung 46170 PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection none, need Cas9 plasmid ...

  8. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... none S. pyogenes Kamoun pUC119-gRNA 52255 Plant PCR template none S. pyogenes Sheen pRGEB31 51295 Plant...none S. pyogenes Boxem PU6::klp-12_sgRNA 46170 Worm PCR (see paper) none S. pyogenes Calarco SP6-sgRNA-scaffold...pyogenes Sternberg pJW1311 61254 Worm none, fusion PCR none S. pyogenes Ward pCFD1-dU6:1gRNA 49408 Fly BbsI...Genome Editing in Monocot and Dicot Plants Plant PCR-based procedure to rapidly generate multiple sgRNA... pyogenes Conklin pcrRNA.ind 61284 Bacteria araB none S. pyogenes Beisel pcrRNA.con 61285 Bacteria J23119...Enzyme Co-expressed Cas9 Cas9 System Selection PI pCRISPR 42875 Bacteria BsaI none S. pyogenes Chloramphenicol...42250 Zebrafish BsaI none S. pyogenes Joung pCRISPathBrick 65006 Bacteria BsaI yes, interfere S. pyogenes...

  9. Plan Your Experiment

    Type
    Collection
    ...methods like sequencing or digital PCR , RNA-based methods like qPCR , and/or protein-based methods like...Very inefficient Can't distinguish specific edits PCR Knockouts Large insertions/deletions Some prime and...provide specific sequence information alone Digital PCR Knockouts Insertions/deletions Prime and base edits...RNA-sequencing, Perturb-seq, deep sequencing, etc. qPCR Knockouts Activation/interference Needs good primer...

  11. Antibody Production

    Type
    Collection
    ...heavy chain and/or light chain variable regions. The PCR product is purified, sequenced via the Sanger method...

  12. CRISPR Plasmids - Plants

    Type
    Collection
    ...BsaI none S. pyogenes Kamoun 52255 pUC119-gRNA U6 PCR template none S. pyogenes Sheen 51295 pRGEB31 rice...

  13. Open Enzyme Collection

    Type
    Collection
    ...this collection include polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription...

  14. TALEN Plasmids and Kits

    Type
    Collection
    ...somatic cells. Keith Joung Zhang Lab TALE Toolbox PCR/Golden Gate cloning method. Optimized for human expression...

  15. CRISPR References and Information

    Type
    Collection
    ...the tool, you'll need Sanger sequencing reads from PCR amplicons that cover your locus of interest and correspond... PDF, 129 KB Marraffini Bacteria: pCRISPR new spacer cloning pCRISPR PDF, 109 KB Mendenhall and Myers ...

  16. Retrograde AAV viral preps

    Type
    Collection
    ...AAV titering by SYBR green qPCR , confirmation of the transfer plasmid with PCR, purity assessment by silver...

  17. Caltech Systemic Capsids

    Type
    Collection
    ...including AAV titering by probe-based droplet digital PCR, in vitro and (when possible) in vivo testing, and...

  19. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...promoters (orange). Hybridoma-derived V L and V H domain PCR products are fused to a joining fragment comprising...translocation of the heavy chain across the ER membrane. PCR-mediated fusion of these three elements is followed...

  20. Immunology Research Plasmids and Resources

    Type
    Collection
    ...PROCR protein C receptor, endothelial (EPCR) CCCA, CCD41, CD201, EPCR, MGC23024, bA42O4.2 PSMB8 proteasome...receptor 2 CYSLT2, CYSLT2R, GPCR, HG57, HPN321, KPG_011, PSEC0146, hGPCR21 DARC Duffy blood group, chemokine...relaxin/insulin-like family peptide receptor 3 GPCR135, MGC141998, MGC142000, RLN3R1, RXFPR3, SALPR SAA1... estrogen receptor 1 CEPR, CMKRL2, DRY12, FEG-1, GPCR-Br, GPR30, LERGU, LERGU2, LyGPR, MGC99678 GPHA2 ...repeat-containing G protein-coupled receptor 6 FLJ14471, GPCR, VTS20631 LHB luteinizing hormone beta polypeptide...relaxin/insulin-like family peptide receptor 3 GPCR135, MGC141998, MGC142000, RLN3R1, RXFPR3, SALPR RXRA...
Showing: 1 - 20 of 33 results