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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 28 results for: pcr

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Showing: 1 - 20 of 28 results

  1. Viral Production

    Type
    Collection
    ... AAV particles are titered by droplet digital PCR (ddPCR) using primers and probes targeting the ITR elements...April 2016) also performed by droplet digital PCR (ddPCR). Quality Control Addgene ensures high quality...are titered using a lentiviral droplet digital PCR (ddPCR) titration protocol (modified from Wang et al...confirmed to have the correct integrated provirus using PCR. This assay involves transducing cells with serial...obtained using ddPCR are generally 2–3 fold higher than those achieved using the standard qPCR method. Titering...

  3. CRISPR Plasmids - Tagging

    Type
    Collection
    ... technique for use in Drosophila cells that uses PCR to generate both an expression cassette for the Cas9.... The plasmids in the following articles provide PCR templates for amplification of the tag (eg GFP, Flag...expresses Cas9, PITCh-gRNA, & FBL-specific gRNA pCRIS-PITChv2-FBL - PITCh donor vector for EGFP-2A-PuroR...

  4. Zhang Lab CRISPR Page

    Type
    Collection
    ... be co-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran et al., Nature Protocols...codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature Protocols 2013). Individual...

  6. Plan Your Experiment

    Type
    Collection
    ...methods like sequencing or digital PCR , RNA-based methods like qPCR , and/or protein-based methods like...Very inefficient Can't distinguish specific edits PCR Knockouts Large insertions/deletions Some prime and...provide specific sequence information alone Digital PCR Knockouts Insertions/deletions Prime and base edits...RNA-sequencing, Perturb-seq, deep sequencing, etc. qPCR Knockouts Activation/interference Needs good primer...

  8. Antibody Production

    Type
    Collection
    ...heavy chain and/or light chain variable regions. The PCR product is purified, sequenced via the Sanger method...

  9. Open Enzyme Collection

    Type
    Collection
    ...this collection include polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription...

  10. TALEN Plasmids and Kits

    Type
    Collection
    ...somatic cells. Keith Joung Zhang Lab TALE Toolbox PCR/Golden Gate cloning method. Optimized for human expression...

  11. CRISPR References and Information

    Type
    Collection
    ...the tool, you'll need Sanger sequencing reads from PCR amplicons that cover your locus of interest and correspond... PDF, 129 KB Marraffini Bacteria: pCRISPR new spacer cloning pCRISPR PDF, 109 KB Mendenhall and Myers ...

  12. Retrograde AAV viral preps

    Type
    Collection
    ...AAV titering by SYBR green qPCR , confirmation of the transfer plasmid with PCR, purity assessment by silver...

  13. Caltech Systemic Capsids

    Type
    Collection
    ...including AAV titering by probe-based droplet digital PCR, in vitro and (when possible) in vivo testing, and...

  15. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...promoters (orange). Hybridoma-derived V L and V H domain PCR products are fused to a joining fragment comprising...translocation of the heavy chain across the ER membrane. PCR-mediated fusion of these three elements is followed...

  16. Immunology Research Plasmids and Resources

    Type
    Collection
    ...PROCR protein C receptor, endothelial (EPCR) CCCA, CCD41, CD201, EPCR, MGC23024, bA42O4.2 PSMB8 proteasome...receptor 2 CYSLT2, CYSLT2R, GPCR, HG57, HPN321, KPG_011, PSEC0146, hGPCR21 DARC Duffy blood group, chemokine...relaxin/insulin-like family peptide receptor 3 GPCR135, MGC141998, MGC142000, RLN3R1, RXFPR3, SALPR SAA1... estrogen receptor 1 CEPR, CMKRL2, DRY12, FEG-1, GPCR-Br, GPR30, LERGU, LERGU2, LyGPR, MGC99678 GPHA2 ...repeat-containing G protein-coupled receptor 6 FLJ14471, GPCR, VTS20631 LHB luteinizing hormone beta polypeptide...relaxin/insulin-like family peptide receptor 3 GPCR135, MGC141998, MGC142000, RLN3R1, RXFPR3, SALPR RXRA...

  17. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...receptors (GPCRs) Yeast GPCR-sensor Toolkit includes plasmids for constructing highly-tunable GPCR-based biosensors...eabq8173. Yulong Li Norepinephrine nLight red and green GPCR-based NE indicators Sensitive multicolor indicators...Tommaso Patriarchi Norepinephrine GRAB_NE family of GPCR activation-based NE sensors A Genetically Encoded...

  18. Brain Initiative Collection

    Type
    Collection
    .... Used for photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV8 .... Used for photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV9 .... Used for photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 140135-AAV1 ...

  20. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's Mark Cookson 29354 pCR8-DJ1-WT PARK7 Parkinson's Mark Cookson 29355 pCR8-DJ1-D149A PARK7 Parkinson's...Parkinson's Mark Cookson 29356 pCR8-DJ1-E64D PARK7 Parkinson's Mark Cookson 29357 pCR8-DJ1-M26I PARK7 Parkinson's...Mizushima 234360 pCR4_miniFXN_7 FXN Flag T7 Friedreich ataxia Marek Napierala 234361 pCR4_miniFXN_8 FXN Flag ...-LFA EIF2AK2 GAL VWM disease Harmit Malik 20123 PCRII-zSynC SNCB Lewy body dementia Aaron Gitler 20648...SOD1 GFP CMV ALS Elizabeth Fisher 26573 CaV2.1 (pCR3.1) CACNA1A CMV Spinocerebellar ataxia 8 Diane Lipscombe...
Showing: 1 - 20 of 28 results