We narrowed to 17 results for: pcr

Protocols for plasmid cloning by PCR.

Description of Polymerase Chain Reaction with protocol, tips and FAQ

Follow this protocol to perform digital droplet PCR (ddPCR) for purified AAV titration.

Follow this protocol to perform digital droplet PCR (ddPCR) for lentivirus vector titration.

Determine the number of genome-containing particles in an AAV prep using qPCR, SYBR Green Technology, and Addgene’s AAV Titration protocol.

Get started with Gibson Assembly Cloning!

Tips on designing primers for PCR

Protocol for Ligation Independent Cloning

A wide variety of protocols from Addgene that can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations.

Protocol for how to purify DNA from an agarose gel, including tips to help improve the resolution and separation of bands.

Modify your plasmid MCS or add short sequences using oligo overlap cloning.

A collection of Addgene's video content, including how-to screen captures, lab procedure protocols, and career advice videos.

Protocol for how to perform a diagnostic digest to verify plasmid size, verify insert orientation, and more.

Protocol, tips, and FAQ for how to perform a restriction digest of plasmid DNA

Protocol - How to Ligate Plasmid DNA

Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human and mouse genes.

Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental procedures.