We narrowed to 103 results for: primer

FastCloning is a fast and easy technique that can replace restriction cloning. Learn about this great plasmid cloning technique here.

Nov. 1, 2016, 2:30 p.m.

Learn how to use recombinase-based state machines from the Lu lab to monitor cellular events and regulate gene expression.

July 28, 2016, 2:30 p.m.

Learn how to use and apply NEBuilder HiFi DNA Assembly Technology from New England Biolabs for quick and accurate plasmid cloning.

Sept. 6, 2017, 1:01 p.m.

This post provides tips for designing a prime editing experiment, from pegRNA design, PE tool selection, and choice of DNA sequence to edit.

Jan. 23, 2025, 2:15 p.m.

Learn how we titer AAV for our viral service at Addgene and find tips for improving your AAV tittering methods.

Nov. 15, 2017, 3 p.m.

Learn how to use in vitro screening and other tricks to expedite the CRISPR genome editing process in mice.

June 28, 2016, 10:59 a.m.

With the limitations of qRT-PCR based COVID-19 testing, scientists develop CRISPR-based diagnostic tools for COVID-19.

May 5, 2020, 1:15 p.m.

Find some tips and tricks for using CRISPR for gene editing.

March 7, 2017, 3:30 p.m.

an overview of qPCR and ddPCR copy number variant assays.

April 26, 2022, 1:15 p.m.

Ever wondered how Addgene makes our viral preps? We explain it all here!

Oct. 24, 2023, 1:15 p.m.

New to plasmids? Learn the basics of plasmids in this blog post and find out about the parts of a plasmid, how they are made, and how scientists can use them in the lab.

April 2, 2020, 6:17 p.m.

Learn how Addgene has worked to increase accessibility to our materials and educational resources.

Nov. 21, 2024, 10:47 p.m.

Addgene is now using NGS services from seqWell Inc for plasmid validation. Learn how the seqWell sequencing process works here.

April 19, 2017, 3:25 p.m.

TOPO cloning is a restriction enzyme and ligase free cloning method. Learn how TOPO works in this post.

Oct. 27, 2016, 2:30 p.m.

Addgene's plasmid kits allow you to order many plasmids for a single purpose at a low price.

Sept. 14, 2016, 2:30 p.m.

Take a look at these 5 great apps to make your lab life easier including tools from Gibco, LabArchives, NEB, ThatsMyStapler, and iGreenEarth.

Sept. 22, 2016, 2:30 p.m.

An end of year list of 12 CRISPR resources available on Addgene, focusing on ones added in 2021.

Dec. 28, 2021, 2:15 p.m.

Check out how you can use digital PCR to validate your CRISPR genome edits.

March 30, 2017, 2:30 p.m.

Prime editing is a search-and-replace CRISPR editing method that offers versatility and precision in genome editing. We cover common prime editing tools.

Aug. 11, 2026, 1:15 p.m.

Mycoplasma contamination? Find out where it comes from, how to detect it, prevent it, and remove it from your tissue culture experiments.

Oct. 8, 2020, 1:15 p.m.