Protocol - How to Design Primers
Type
Protocol
Description
Tips on designing primers for PCR
We narrowed to 12 results for: primer
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What is Polymerase Chain Reaction (PCR)
TypeProtocolDescriptionDescription of Polymerase Chain Reaction with protocol, tips and FAQ -
Protocol - How to Perform Sequence Analysis
TypeProtocolDescriptionBest practices for performing sequence analysis of a plasmid -
Plasmid Cloning by PCR (with Protocols)
TypeProtocolDescriptionProtocols for plasmid cloning by PCR. -
Gibson Assembly Protocol
TypeProtocolDescriptionGet started with Gibson Assembly Cloning! -
Ligation Independent Cloning
TypeProtocolDescriptionProtocol for Ligation Independent Cloning -
AAV ddPCR Titration
TypeProtocolDescriptionFollow this protocol to perform digital droplet PCR (ddPCR) for purified AAV titration. -
Lentivirus ddPCR Titration
TypeProtocolDescriptionFollow this protocol to perform digital droplet PCR (ddPCR) for lentivirus vector titration. -
AAV Titration by qPCR Using SYBR Green Technology
TypeProtocolDescriptionDetermine the number of genome-containing particles in an AAV prep using qPCR, SYBR Green Technology, and Addgene’s AAV Titration protocol. -
Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps
TypeProtocolDescriptionA wide variety of protocols from Addgene that can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations. -
Plasmid Modification by Annealed Oligo Cloning (with Protocols)
TypeProtocolDescriptionModify your plasmid MCS or add short sequences using oligo overlap cloning. -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocolDescriptionAddgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human and mouse genes.