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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8 results for: primer

Showing: 1 - 8 of 8 results

  1. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...sequences for each (these can be built into PCR primers when creating a new part, with an additional 5'...suffix will need to be reverse complimented for primer design. The restriction enzymes used for assembly...

  2. Viral Production

    Type
    Collection
    ...are titered by droplet digital PCR (ddPCR) using primers and probes targeting the ITR elements and an internal...incubated for 72 h, and genomic DNA is extracted. Primers and probes targeting integrated copies of the lentiviral...

  3. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...protothropic cassette can be amplified with the following primers: FW 5'–GGTGACGGTGCTGGTTTA–3' RV 5'–TCGATGAATTCGAGCTCG...

  4. Antibody Production

    Type
    Collection
    ...residual plasmid is extracted and amplified with primers targeting the heavy chain and/or light chain variable...

  5. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...the target site. The longer pegRNA also encodes a primer binding site (PBS) and the desired edits on an ...

  7. Plan Your Experiment

    Type
    Collection
    ...qPCR Knockouts Activation/interference Needs good primer design Western blot Knockouts Activation/interference...

  8. CRISPR Guide

    Type
    Collection
    ...flap. However, the longer pegRNA also encodes a primer binding site (PBS) and the desired edits on a RT...gRNA; a specialized gRNA containing an additional primer binding site and reverse transcription template...
Showing: 1 - 8 of 8 results