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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5 results for: primer

Showing: 1 - 5 of 5 results

  1. Sequencing Primers

    Type
    Guide
    ...what primers are listed under "5' sequencing primer" and "3' sequencing primer" or explore the sequence ...list of universal sequencing primers and other common sequencing primers. Educational...Molecular Biology Reference Sequencing Primers Sequencing Primers Though Addgene mainly uses next-generation...sequencing primers. These lists are provided for your reference, Addgene does not distribute primers. To identify...additional annotated primers. You can also use Addgene’s protocol to design your own primers . Still have questions...plasmid verification, Addgene has used a number of primers for Sanger sequence verification over the years...years. Below is a list of popular universal primers available from most sequencing services, along with a...

  2. Molecular Biology Reference

    Type
    Guide
    .... For a list of commonly used primers, check out our sequencing primers page . Types of Plasmids Plasmids...enzyme, the template DNA to be copied, and a primer. A primer is a small piece of DNA, approximately 18–...Molecular Cloning Techniques Promoters Sequencing Primers Origins of Molecular Genetics The concept of genes...sort the cells by visualization or flow cytometry. Primer Binding Site A short, single-stranded DNA sequence...amplification or DNA sequencing of the plasmid. Primers can be utilized to verify the sequence of the insert...know some of its sequence to design a effective primer. To learn more about the sequencing technologies...approximately 500–1,000 bases downstream of the known primer region with very few errors, making it an efficient...

  3. Molecular Cloning Techniques

    Type
    Guide
    ...Complementary overhangs are built into the PCR primers for the insert, based on the destination vector...Additional Resources Addgene Webpages Sequencing Primers Modular Cloning (MoClo) Guide Choosing Your Perfect...

  4. Plan Your Experiment

    Type
    Guide
    ...qPCR Knockouts Activation/interference Needs good primer design Western blot Knockouts Activation/interference...

  5. CRISPR Guide

    Type
    Guide
    ...flap. However, the longer pegRNA also encodes a primer binding site (PBS) and the desired edits on a RT...gRNA; a specialized gRNA containing an additional primer binding site and reverse transcription template...
Showing: 1 - 5 of 5 results