We narrowed to 103 results for: primer

Learn about key considerations when conducting optogenetics experiments in mice, including how to pick and delivery an opsin and stereotaxic surgery tips.

Sept. 10, 2015, 2:30 p.m.

Find tips on how to perform both large and small scale plasmid verification and sequencing.

Jan. 14, 2016, 3:30 p.m.

Colony PCR is a useful technique for quickly screening for successful constructs after a plasmid cloning experiment.

May 12, 2016, 2:30 p.m.

PCR can be used to append the appropriate restriction sites to your insert and facilitate downstream cloning. Read on for tips on this process.

March 29, 2016, 2:30 p.m.

In Vivo Assembly (IVA) cloning uses the bacterial recombination pathway to allow any cloning procedure to be performed with a simple two-step, 2 hr protocol prior to transformation. This post covers the basics of the IVA cloning procedure, primer design, and tips & tricks for successful IVA cloning.

Oct. 18, 2018, 12:37 p.m.

A deep dive into qPCR with a focus on analysis, chemistries, and bench tips.

Sept. 29, 2022, 1:15 p.m.

A list of free online molecular biology tools for plasmid mapping, DNA and protein sequence analysis, primer design and more.

Sept. 12, 2023, 1:15 p.m.

Here, we cover four different plasmid screening strategies including blue-white screening, colony PCR, restriction digest analysis, and Sanger sequencing.

Sept. 15, 2020, 1:15 p.m.

Use site directed mutagenesis to insert small mutations into your plasmid of interest and follow these tips for a smooth process with easy validation.

Aug. 2, 2016, 2:30 p.m.

The basics of PCR covering end point PCR, qPCR, ddPCR, and multiplex PCR and how each method is used.

Aug. 10, 2021, 1:15 p.m.

One-pot saturation mutagenesis can quickly generate libraries of mutant plasmids for phenotypic screening. Learn more from SciComm intern Beth Kenkel.

Feb. 22, 2017, 3:30 p.m.

REPLACR-mutagenesis is a new in vivo method for mutating your plasmid constructs. Learn how REPLACR stacks up against other mutagenesis techniques.

Feb. 10, 2016, 3:30 p.m.

Learn how Addgene uses SnapGene to aid in plasmid quality control and check out some tools you might find useful for your own plasmid verification processes.

Feb. 1, 2018, 3:07 p.m.

In this troubleshooting guide, find step-by-step tips and tricks for troubleshooting your cloning experiment.

Sept. 24, 2019, 12:55 p.m.

A new open source tool for CRISPR experiments, CrispyCrunch, has been developed by the Chan Zuckerberg Initiative to design batch gRNAs, templates, and sequencing primers for CRISPR + HDR experiments.

Feb. 7, 2019, 2:16 p.m.

We've partnered with SnapGene to update our plasmid maps. Learn how to use our new maps to accelerate your research.

June 29, 2017, 1:06 p.m.

Gibson Assembly is a popular cloning alternative to traditional restriction/ligation methods. Learn more about this technique here.

March 1, 2016, 3:30 p.m.

Learn about antibody engineering, including what it is and why we like it. Deep dive into antibody directed evolution techniques, and learn about Addgene's partnership with the Institute for Protein Innovation (IPI).

Aug. 5, 2025, 1:15 p.m.

High schoolers invented a new diagnostic for Lyme disease. In the process, they also created a tool for jointly designing RPA primers and Cas12a sgRNAs.

May 12, 2026, 1:15 p.m.

It's important to determine how much functional virus you have a in your prep before starting a transduction. Learn how to titer your lentivirus here.

March 15, 2016, 2:30 p.m.