We narrowed to 296 results for: promoter

Learn about the top requested AAV from 2017: pmSyn1-EBFP-CRE, a modular tool for expressing Cre-recombinase in neurons.

Jan. 17, 2018, 2:57 p.m.

The Addgene repository has many amazing plasmids used by labs all over the world, but what plasmid was request most in 2015?

Jan. 11, 2017, 8:47 p.m.

Drive-and-proccess (DAP) arrays are a new tool from the Gao lab that allows for up to 31-loci CRISPR multiple base editing.

July 7, 2022, 1:15 p.m.

Adeno-associated Virus (AAV) is used extensively for in-vivo transgene expression. Learn how to use AAV in this post.

May 30, 2023, 1:15 p.m.

Description of the components of the 4 most common viruses - AAV, adenovirus, lentivirus, and gamma-retrovirus.

July 25, 2023, 1 p.m.

This post provides an overview on how transcription terminates in prokaryotes and eukaryotes.

March 31, 2016, 2:30 p.m.

Learn about key considerations when conducting optogenetics experiments in mice, including how to pick and delivery an opsin and stereotaxic surgery tips.

Sept. 10, 2015, 2:30 p.m.

Looking for a way to test a lot of different parts in your cloning, or maybe interrogate some complex genetic circuits? MoClo just might be the approach for you!

March 12, 2024, 1:15 p.m.

With it's small crRNAs and RNase activity, Cpf1 can easily be used for multiplex genome editing. Learn more about new multiplex Cpf1 tools here.

May 9, 2017, 2:12 p.m.

Learn about new plasmids at Addgene including CRISPRa in bacteria, voltage indicators, CRISPR knockout library for mice, and lipoprotein tools.

Dec. 10, 2019, 1:58 p.m.

Using synthetic biology principles has simplified and improved plasmids for optogenetic control of gene expression in E. coli.

Sept. 8, 2015, 2:30 p.m.

History, composition, and tips for lentiviral vectors all in one convenient blog post.

March 21, 2017, 2:30 p.m.

Read on for a sampling of some of the exciting new plasmid technologies that have come through our doors in 2015.

Dec. 23, 2015, 3:30 p.m.

Green fluorescent protein (GFP) and its derivatives can be used experimentally as fusion tags, biosensors, reporters, and more.

May 15, 2014, 3:33 p.m.

Learn how to use recombinase-based state machines from the Lu lab to monitor cellular events and regulate gene expression.

July 28, 2016, 2:30 p.m.

Lambda Red mediated recombineering can be used to generate point mutations in plasmids and bacterial genomes. Learn how to use Lambda Red in this post.

Dec. 15, 2016, 3:57 p.m.

Discover the tools you need to perform plant genome engineering with CRISPR.

Oct. 11, 2016, 2:30 p.m.

Restriction cloning is the cloning technique most molecular biologists start off with. Learn the ins and outs of restriction cloning here.

Feb. 18, 2016, 3:42 p.m.

Stochastic multicolor labeling is a popular technique to label individual cells with different colors and allows for lineage tracing of cell populations over time. Read this blog post to learn about the different systems for various organisms.

March 5, 2019, 1:08 p.m.

Learn about the parts of the AAV RepCap helper plasmid and how they help to package AAV.

Dec. 3, 2020, 2:15 p.m.