...a modern e-commerce company and establishing credibility Chapter 5 - Realizing that Addgene would be successful...plasmid distribution!) Chapter 8 - Opportunities to create repositories with other materials Chapter 9 - The...Addgene, Kenneth was an investment banking analyst at Credit Suisse’s Technology Group in Palo Alto, CA.  Benjie...

...publication. Accessibility is surely also behind the increase in citations for older publications. (As an interesting...most cited. And, as scientific discourse has increasingly moved online, surely times have changed. Altmetrics.org...what impact looks like and what it’s made of. Increasingly, journal publications alone aren’t enough. Scientists...

...take this initiative to the next level with the creation of the The Professional Development Hub (pd|hub...drive change. This effort is already resulting in concrete actions and outcomes. For example, pd|hub assembled...collections of evidence-based practices. These concrete programs and practices to promote PhD professional...

... to stress altogether. Their muscles showed an increase in inflammatory cytokines as a result of those...turns out that activation of the PGC-1a1 pathway increases skeletal muscle expression of kynurenine aminotransferases... proteins and metabolites that are dynamically secreted by muscle during exercise to meet the brain’s ...

... in order to do the thing you love, you have to create opportunities. If you find yourself asking, "Why...position exist yet?", you are in the early stages of creating an opportunity. That's how Addgene was founded...That's how my position and my team at Addgene was created.”- Eric Perkins, Director of Science Product Management...

...optogenetic controls, gene expression levels reliably increased in vitro by a factor of 10 to 20. A flexible and...different effectors. I’d encourage people to get creative with them for their own uses.” Konermann said ...found here.) So tell us, readers, how will you get creative with LITE? Reference: Silvana Konermann et al...

...longer allowing myself to just simply exist without creating a storm. Given all the options out there, I was...the company. One of the things I wanted to do was create content that was unique but also brand friendly... a new design program, Canva, and I was able to create cute and on-brand templates for these posts. ...

... developed SECURE cytosine base editors, which decrease the rate of off-target genome edits compared to...adenine and cytosine editor). Target-ACE exhibits increased editing efficiency of a target site compared to...advantages like reduced off-target activity and decreased immune response to Cas9. Recently, Baisong Lu’...

...million in annual R&D expenditures to support increased public access to the results of federally-funded...recommend Eric Lander's intro to biology course "The Secret of Life." 10)     And, last but not least (certainly...know your favorite ways to share science. Image credit: Judy Breck, via flickr  ...

... we created one long lunch table and we eat together Harry Potter-style.The shared lunches create a culture...or says something about my cooking.   This is a creative (and delicious) team building activity that is...

...characterized, it turns out this protein has a few more secrets - during a collaboration, members of Maureen Hanson...necessary to observe a signal. Red signal intensity increases with GFP concentration and saturates around 30...likely to be most useful for labs that already have created a transgenic GFP-containing organism,” says Hanson...

...holds yearly planning meetings with each trainee to create a supportive, growth-oriented lab culture. The ...perfectionists,” and we don’t often give ourselves credit for what we have accomplished. Starting with a ...meetings make the lab run more smoothly. With a concrete plan in hand, trainees are motivated to complete...

...that your protein might not fold well, you should screen a few different vectors to see which gives you ...subtilis and the yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful ...peptide (SP) that routes the protein through the secretory pathway to improve folding and disulfide bond ...

... today. Although I wish I could take all of the credit, the success of the company is attributed to the... of an interactive game that Addgene employees created and designed for the Hall of Human Life at the ...Addgene means to them. Ken mentioned Addgene’s incredible growth and our determination to keep materials...

...Use software such as Evernote or Google Tasks to create lists of your upcoming tasks, set deadlines, and... computer or smartphone. Evernote allows you to create notes directly from webpage clippings, photographs...attachments. Organize your notes into notebooks, create checklists that link to other notes, and use the...

... interacting partners and perform whole animal screens for small molecules that block the neurodegenerative...their expression in specific cells. With a more concrete understanding of the molecular biology behind ...and pathways to target in future small molecule screens. He plans to work with a partner at IRCCS Besta...

...response to the cell cycle or other stimuli.   To create a colorful Cas9 toolbox, Ma et al. turned to SpCas9...was fused to a different fluorescent protein to create three colors. The specificity of these orthologs...nucleome a specific priority, as researchers work to increase our understanding of chromatin organization. This...

... the connection between LEGOs and science more concrete to help further unlock the potential contained...process One major challenge when we started was creating a LEGO stage that resembles a real laboratory.... your experiments to think outside the box, be creative, and put your inner child to work. If you have...

...The last trick GeoCas9 has up its sleeve is its increased longevity in human serum. Harrington et al suspected...shown that a cocaine esterase engineered to have increased thermostability also had a longer lifetime in ...Harrington, Lucas, et al. "A thermostable Cas9 with increased lifetime in human plasma." bioRxiv (2017): 138867...

...plasmid analysis tool including editing, annotating, creating maps, and more. This tool is maintained by M. ...University of Utah.   Figure 1: A plasmid map created in SnapGene of Blue Flame plasmid pET21a-BirA. ...repository for Cas enzymes.  Open repository of CRISPR screens Cellosaurus: A knowledge resource for cell lines...