We narrowed to 690 results for: eng

...comments section below! Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.       Resources...

...scientists and technical people) is linked to engagement and access to growth opportunities, not salary...zone when you lose track of time because you are engrossed in a task. Things seem to fall into place frequently...

...specific behaviors. Chemogenetics uses genetically engineered receptors to interact with small molecules to...Designer Drugs (DREADDs) (Roth, 2016). DREADDs were engineered from members of the human muscarinic receptor...

...Finally a set of wild type and codon optimized, full length and truncated versions of SARS-CoV-2 S (spike protein...requested the plasmid. By leveraging our core strengths of intensive screening and the use of high throughput...

...CRISPR-Cpf1 genome editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex...spCas9: ~4 kbsaCas9: ~3 kb ~3.9kb crRNA/gRNA length gRNA: ~100 nt crNA: ~42 nt Requires tracrRNA?...

...Even if you're currently a synthetic biologist engineering E. coli metabolism, maybe you’ll be able to apply...Optogenetics, Neuroscience, and the Future of Neuroengineering Primer on Optogenetics Resources at Addgene.org...

...movement is that it provides interesting and unique challenges for intellectual property lawyers. While there...be, an interesting legal and professional challenge.Paratore: The promise there is the ability share the...

...utilizes a gRNA which is photocaged and unable to engage a target sequence. Upon a brief light exposure ...Rosenberg, M., Hendel, A. Using Synthetically Engineered Guide RNAs to Enhance CRISPR Genome Editing Systems...

...expression of R-mAbs is that it can easily be engineered. Like humans, mice express multiple subclasses... Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. A toolbox of IgG subclass-switched...

...scientific paper, you may feel it’s overwhelming or challenging. So instead, let’s focus on practicing with a...dive into applying these four steps elsewhere, engaging in this practice, and perhaps modifying it as ...

...can I use it? CasPEDIA operates like many search engines, featuring both a general search bar tool and an...      CRISPRpedia IGI’s digital resources in English/Spanish  ...

...Campus and the University of California, Berkeley engineered an AAV variant that efficiently infects neurons...recombinase-dependent vectors, this can cause challenges for the experimental design as these vectors ...

...developed a new calcium indicator, OCaMP. The challenge: Expanding the fluorescent toolbox Modern neuroscience...selecting a fluorophore matched to their excitation wavelength. OCaMP is well-suited for these lasers, with ...

...asked questions about γ-retroviral vectors How do engineered γ-retroviruses differ from lentiviruses? There...There are a few important differences between engineered γ-retroviruses and lentiviruses. First, these viruses...

...typically their own genome, but this step can be engineered to be anything you want delivered to your target...and virus. Viral CRISPR-Cas9 tools have been engineered to introduce frameshift mutations in genes of...

...routine, open-source software projects that actively engage with the community are particularly successful....hardware, this process is often much more involved. Challenges such as the provision of an accessible supply...

...SciComm by doing SciComm Writing had always been a strength of Susan’s, but she didn’t know if she liked science...finding that uninterrupted time to be creative is challenging,” says Susan.  Another difficulty is that some...

...the utilization and development of genetically engineered components, like protein tags (Figure 2). Through...Tags Additional Resources on addgene.org New England Biolabs Cell-Imaging Plasmid Collection Promega...

...fragment, Fvs are often produced recombinantly and engineered to be joined by a linker peptide, resulting in...Tomisaki M, Kiuchi T, Takagi J, Watanabe N (2022) Engineered fast-dissociating antibody fragments for multiplexed...

...very pure sample. Because binding strength is proportional to the length of the tag, with longer tags you...