962 results

This blog post explores how commonly used GFP variants can undergo Green-Red photoconversion.

Aug. 4, 2015, 2:30 p.m.

Interview with Professors Eric Mendenhall and Richard Myers about their CRISPR- based system for tagging any endogenous gene of interest.

July 28, 2015, 2:30 p.m.

Makerspaces provide a place for self-directed research. Learn how members of a Makerspace in Victoria Canada model worm locomotion.

July 23, 2015, 2:30 p.m.

Angela Depace's lab recently described how Individual development plans (IDP's) help advance their research. Read on to learn more about IDPs.

July 21, 2015, 2:30 p.m.

Developments in light sheet fluorescence microscopy (LSFM) and tissue clearing enable researchers to visualize tissues of transgenic and non-transgenic organisms in 3D.

July 16, 2015, 2:30 p.m.

It pays for scientists to have a great resumes and CVs. Follow these simple steps to help get you and your resume noticed by future employers.

July 9, 2015, 2:30 p.m.

This blog post describes a a self-excising cassette that can be used with CRISPR/Cas9 tools in C. elegans to generate genomic modifications.

July 7, 2015, 3:36 p.m.

Learn how Tyler J. Ford, an Outreach Scientist at Addgene, transitioned from graduate school to working at Addgene.

July 2, 2015, 8:37 p.m.

Overview of the Sleeping Beauty transposase system and how they are efficient tools for genome engineering.pl

June 30, 2015, 2 p.m.

iTOP is a protein-only transduction method for delivering proteins and RNA into a variety of cell types, with potential applications with CRISPR/Cas9.

June 23, 2015, 8:37 p.m.

The pCri plasmids available through Addgene allow researchers to multiplex the protein purification process saving time and frustration.

June 19, 2015, 3:08 p.m.

Science in the News (SITN), a Harvard group dedicated to making science understandable, recently hosted DayCon, a science conference for the general public.

June 17, 2015, 9:04 p.m.

Addgene plasmid pages now show whether a plasmid has been cited in other articles. Use the "References" link to view the articles citing your plasmid.

June 16, 2015, 2:15 p.m.

Blue-white screening is a well loved technique that allows scientists to quickly identify recombinant clones for further analysis.

June 4, 2015, 1:03 p.m.

Overview of polycistronic glycine tRNA-gRNA (PTG) multiplexing gRNA cassettes, with comparisons to Pol III and Cys4 gRNA cassettes.

June 2, 2015, 6:06 p.m.

This blog posts discusses Cas9 gene drives and the appropriate safeguard and confinement strategies scientists should use for these gene drives.

May 22, 2015, 4:58 p.m.

Overview of recombinases that can be used as a Cre-alternative for site-specific recombination in Drosophila.

May 12, 2015, 1:32 p.m.

Tips for writing your scientific manuscript, including how to search for and organize papers.

May 5, 2015, 3:54 p.m.

An overview of the Brainbow system and its variants and how they utilize Cre-lox for multicolor labeling of neurons.

April 24, 2015, 2:39 p.m.

Addgene's interviews Chris Voigt of MIT about the intersection of CRISPR and synthetic biology and his new work building circuits with CRISPRs.

April 22, 2015, 2:06 p.m.

Learn more about non-homologous end joining (NHEJ) and how it relates to genome engineering technologies such as CRISPR/Cas9.

April 16, 2015, 3:45 p.m.

Controls are required for any plasmid-based experiment in order to accurately assess the results. This blog posts reviews different types of control plasmids, including but not limited to internal controls, negative controls, positive controls, and replicate controls.

April 9, 2015, 3:29 p.m.

Overview of the CRISPRi system for rewiring metabolic circuitry, with a focus on the paper by Zalatan et al 2015.

April 7, 2015, 4:21 p.m.

As part of Addgene's Management for Scientists, this post describes the differences between managing vs. leading and the skills associated with each.

March 31, 2015, 4:08 p.m.

Learn about how Addgene has shipped over 500,000 plasmids and where those plasmids go.

March 26, 2015, 3:24 p.m.

Learn more about the Developmental Studies Hybridoma Bank (DSHB), a repository for monoclonal antibodies, that distributes antibodies and their associated hybridoma cell lines.

March 10, 2015, 2:25 p.m.

Read about artists who are turning science into art by using scientific techniques to view the world from another vantage point.

March 2, 2015, 3:13 p.m.

New CRISPR pooled libraries, such as SAM, CRISPRa, and CRISPRi, offer new ways to do genome-wide functional screens.

Feb. 24, 2015, 7:50 p.m.

Learn how effective delegation allows you to make the best use of you and your team's time and skills in this Management for Scientists post.

Feb. 20, 2015, 8:30 p.m.

Hear from the authors about the generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9.

Feb. 18, 2015, 3:09 p.m.

Learn more about E. coli strains used for protein overexpression.

Feb. 10, 2015, 3:06 p.m.

Focusing on the importance of giving feedback to your employees, this is the third installment of a 5 part series on Managing People for Scientists.

Feb. 6, 2015, 2:46 p.m.

Addgene data on CRISPR and synthetic biology plasmids deposited to the repository and distributed around the world.

Feb. 4, 2015, 3:58 p.m.

What is a career coach and how can scientists find the right one for them?

Jan. 29, 2015, 1:25 p.m.

Dora Farkas, career coach for scientists, discusses how to identify difficult supervisors and outlines 7 strategies to resolve conflicts.

Jan. 21, 2015, 1:30 p.m.

Overview of the 2015 Keystone Symposium: Precision Genome Engineering and Synthetic Biology from Addgene's own Eric Perkins, including the state of CRISPR.

Jan. 15, 2015, 1:50 p.m.

Overview of Cre-lox recombination and how it is used to make DNA modifications in transgenic animals, embryonic stem cells, and tissue-specific cell types.

Jan. 13, 2015, 3:47 p.m.

Addgene's Executive Director, Joanne Kamens, shares advice for management of scientists on how to seek feedback from your employees.

Jan. 8, 2015, 2:34 p.m.

22 of the interesting and popular hot plasmid technologies of 2014 from Addgene's plasmid repository.

Jan. 6, 2015, 5:21 p.m.

Tips for preparing and writing a resume when transitioning from an acamedic to a non-academic science career.

Dec. 18, 2014, 4:06 p.m.