We narrowed to 623 results for: Dos
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TypeBlog PostPublishedDec. 19, 2017, 2 p.m.... crucial skill for career advancement. Scientists do and should tell stories all the time. They provide...
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Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog PostPublishedSept. 15, 2016, 2:30 p.m.... know, treatments that work well in animal models do not always work as efficiently in humans. This is... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog PostPublishedJan. 15, 2015, 1:50 p.m....and excitement about how much more there is left to do with the characterization and implementation of this... -
Choosing Your Perfect Empty Backbone
TypeBlog PostPublishedAug. 19, 2014, 3:39 p.m....track your protein in the cell or purify it – and you do not have a specific antibody against the protein ... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog PostPublishedJan. 30, 2014, 3:29 p.m....vary in their sensitivitly to heat and/or light – do not add them to media hotter than about 55oC and ... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog PostPublishedJan. 21, 2014, 3:08 p.m....education and mastering new skill sets must occur by doing work outside of the lab. Extracurricular activities... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog PostPublishedDec. 17, 2020, 1:30 p.m....efficient editing. Repair oligos do not need to be PAGE purified, although doing so increases knock-in efficiency... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog PostPublishedJuly 19, 2016, 2:30 p.m....cofactor? How many binding partners does it have and how do they come off and on in a cellular environment? If...can undergo FRET when they are near each other. How do you label your biomolecule of interest with FRET ... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog PostPublishedSept. 10, 2015, 2:30 p.m....most important question to consider is what exactly do you want to use optogenetics for? The technique is... is non-trivial; you don’t want to spend months doing surgeries and making fiber optic cables only to ... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog PostPublishedSept. 3, 2019, 1:15 p.m....eukaryotes don’t naturally have this response, they do contain the specific ubiquitin ligase pathway needed... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m....read of the paper is much more detailed. I prefer to do it on another day - preferably the following morning... -
The MTA at Addgene
TypeBlog PostPublishedJuly 23, 2024, 2:17 p.m....transparency in an otherwise messy process. And you do have options for the terms you want to share your... -
Antibodies 101: Epitope Tags
TypeBlog PostPublishedMay 11, 2023, 1:15 p.m....- but for many other proteins, quality antibodies do not exist, or they don’t exist for a specific application... -
Antibodies 101: Chimeric Antibodies
TypeBlog PostPublishedApril 25, 2023, 1:15 p.m....indirectly probed simultaneously on the sample. What to do? If you’ve ever found yourself facing a similar ... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....main tenets of our quality control (QC) mantra is to do our utmost to ensure that scientists are receiving... -
Trading Spaces - Visiting Scientist Edition
TypeBlog PostPublishedAug. 22, 2023, 1 p.m....third parties or your home institute. Discuss rights Do you expect to generate data that will contribute ... -
Creating accessible biology activities in schools with BioBits
TypeBlog PostPublishedAug. 2, 2018, 12:56 p.m....also see how fun biology can be! What can BioBits do for you? We initially developed the BioBits kit to... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m....For every task, we envision what the user wants to do and make the path to accomplishing their goals as... -
Visualizing Translation at the Single Molecule Level
TypeBlog PostPublishedAug. 1, 2017, 1:15 p.m....experiments, it’s important to verify that these changes do not impact translation rate, as the authors of these... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog PostPublishedJuly 27, 2017, 1:11 p.m....oligomerize when exposed to ultraviolet light. To do so, she generated fusion proteins in which Dronpa...