We narrowed to 634 results for: Dos

...where affinity tags come in – they are peptides which do not naturally exist in most expression systems that...

...both bioinformatics tools and knowledge databases. Do you have a favorite online molecular biology tools...

...able to detect extracellular stimuli. One way they do so is through G protein-coupled receptors (GPCRs)...

...enveloped viruses bud out, instead of lysing, but they do generally still kill the infected cell.) This is ...

...EasyTag System is a straightforward workflow for doing gene targeting, reporter generation, controllable...

... crucial skill for career advancement. Scientists do and should tell stories all the time. They provide...

... know, treatments that work well in animal models do not always work as efficiently in humans. This is...

...and excitement about how much more there is left to do with the characterization and implementation of this...

...track your protein in the cell or purify it – and you do not have a specific antibody against the protein ...

...vary in their sensitivitly to heat and/or light – do not add them to media hotter than about 55oC and ...

...education and mastering new skill sets must occur by doing work outside of the lab. Extracurricular activities...

...efficient editing. Repair oligos do not need to be PAGE purified, although doing so increases knock-in efficiency...

...cofactor? How many binding partners does it have and how do they come off and on in a cellular environment? If...can undergo FRET when they are near each other. How do you label your biomolecule of interest with FRET ...

...most important question to consider is what exactly do you want to use optogenetics for? The technique is... is non-trivial; you don’t want to spend months doing surgeries and making fiber optic cables only to ...

...eukaryotes don’t naturally have this response, they do contain the specific ubiquitin ligase pathway needed...

...read of the paper is much more detailed. I prefer to do it on another day - preferably the following morning...

...main tenets of our quality control (QC) mantra is to do our utmost to ensure that scientists are receiving...

...transparency in an otherwise messy process.  And you do have options for the terms you want to share your...

...third parties or your home institute. Discuss rights Do you expect to generate data that will contribute ...

...- but for many other proteins, quality antibodies do not exist, or they don’t exist for a specific application...