We narrowed to 675 results for: plasmid
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TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....Fluorescent Protein Resources Find Other Plasmids that Glow (Luciferase Plasmids) Find Optogenetics Resources ...tag with a serious drawback. The standard EGFP plasmid that used to be sold by Clontech and is in a freezer...
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Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....compared to transgenic animals (17). These viral plasmids are available through Addgene. The combination...University of Miami Miller School of Medicine. Find plasmids from the Lee Lab here. Pantelis Tsoulfas is an...University of Miami Miller School of Medicine. Find plasmids from the Tsoulfas lab here. References: H... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog PostPublishedJan. 16, 2025, 3:42 p.m...., et al., 2006). AAV2 capsids are available as plasmids in the repository and can be selected as a serotype..., et al., 2006). AAV8 capsids are available as plasmids in the repository, as part of our in-stock viral...retinal cells (Issa et al., 2023). AAV9 packaging plasmids are available at Addgene. AAV9 vectors are not...Request Browse all in-stock AAV preps Browse all AAV plasmids ... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m.... about FLEx vectors Download our Plasmids 101 eBook Read our plasmids blog posts Resources on the Addgene...molecular biology reference Find Cre-lox plasmids at Addgene Browse plasmids at Addgene ... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog PostPublishedOct. 27, 2020, 1:15 p.m....for mammalian RNAi Browse all plasmids for RNAi Check out Addgene's plasmids and resources for plant biology...harvested instead of microscopic isolation. The plasmids required to conduct an APT-based RNAi experiment... target sequence, as well as essential control plasmids. Many thanks to our guest blogger Robert Orr... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog PostPublishedJan. 13, 2025, 9:49 p.m....this evolving field! Find prime editing plasmids! Find plasmids from David Liu's lab! This post was originally...the CRISPR guide Find CRISPR plasmids by function Browse our entire plasmid collection ...for treating genetic diseases. In our summer Hot Plasmids post, we covered the Liu lab’s first demonstration... -
Year of the Camelid: Antibody Style
TypeBlog PostPublishedMay 9, 2024, 1:15 p.m.... 12–15 kDa Ease of cloning and expression from plasmids, due to only needing one open reading frame Ability...Membrane Proteins More resources from addgene.org Plasmids encoding nanobodies at Addgene Sybody Generation... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog PostPublishedFeb. 13, 2018, 2:51 p.m....protocols Browse CRISPR plasmids for isolating a genomic locus Browse FRET plasmids for studying protein ... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog PostPublishedMay 21, 2020, 1:15 p.m....the upstream recombination outcome. Find SPARC plasmids at Addgene To more precisely control the amount...Resources on Addgene.org Find fluorescent protein plasmids at Addgene Read our Molecular Biology Reference... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog PostPublishedFeb. 14, 2019, 2:35 p.m....other scientists save resources by sharing plasmids Find plasmids at Addgene ... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog PostPublishedSept. 14, 2017, 12:40 p.m....you want to give GeoCas9 a try, as always, the plasmids are available from Addgene. Happy CRISPRing! ...Background with Addgene’s CRISPR Guide Page Find CRISPR plasmids at Addgene ... -
10 Great Guest Posts We're Thankful For
TypeBlog PostPublishedNov. 18, 2016, 3 p.m....Nathaniel Roquet from the Lu Lab at MIT developed plasmid based systems that report the order in which specific... plasmid... -
Using Addgene's New Viral Service for Your Research
TypeBlog PostPublishedNov. 17, 2016, 3:30 p.m....accelerate your research. Why distribute virus? Plasmid distribution is booming, but, over the years, many...list of what’s available or suggest additional plasmids with the Viral Service Suggestion Form. Lentivirus... -
Viral Vectors 101 eBook 2nd Edition
TypeBlog PostPublishedJuly 11, 2024, 1:15 p.m....on the Addgene blog CRISPR 101 eBook en español Plasmids 101 eBook 4th edition Resources on addgene.org... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog PostPublishedJuly 7, 2020, 1:15 p.m....efficiency of plasmid delivery to a cell. In contrast, TREE provides a read out for plasmid delivery and...Addgene.org Browse the CRISPR guide Find CRISPR plasmids for your research ... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....capabilities of full-length SpCas9. This set of plasmids includes AAV-Cas9C-VPR for targeted gene activation...bacterial immune system cleaves bacteriophage and plasmid DNA. Nature 468:67–71 . https://doi.org/10.1038...Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids ... -
Welcome to the Lab Bench! New Protocol Series Helps Trainees Get Started in the Lab
TypeBlog PostPublishedSept. 7, 2021, 1:15 p.m....learning due to COVID-19. We had many protocols for plasmid cloning and working with viral vectors, but what... -
An Addgene Summer Internship
TypeBlog PostPublishedAug. 8, 2024, 7:22 p.m....something wrong. My capstone project was based on plasmid cloning and I, the researcher, got to choose what... we found with the correct bands was the right plasmid, so after discussion, we sent it for sequencing... -
Viral Vectors 101: Transductions
TypeBlog PostPublishedApril 30, 2024, 1:15 p.m....typically transfected along with viral packaging plasmids into a viral production cell line, usually HEK...HEK-293 cells. When all of these viral plasmids are expressed within the production cell line, viral particles... -
FPbase: A new community-editable fluorescent protein database
TypeBlog PostPublishedMay 16, 2018, 1 p.m....Addgene.org Browse all fluorescent protein plasmids Find plasmids for FRET Check out the Michael Davidson...