We narrowed to 706 results for: plasmid

CRISPR-Cas9 technology appears in numerous publications, including its use for genome editing in monkeys and x-ray crystallography of Cas9's 3-D shape.

March 11, 2014, 5:55 p.m.

Learn how to target donor and acceptor fluorophores for FRET experiments.

July 19, 2016, 2:30 p.m.

Learn about OCaMP, a new orange genetically encoded calcium indicator .

Aug. 19, 2025, 1:15 p.m.

Considering purifying a protein to study it? Learn all about the polyhistidine tag, or HIS, in this blog post!

March 2, 2023, 2:15 p.m.

Learn how you can visualize newly synthesized proteins with FUNCAT-PLA.

Jan. 16, 2018, 3:20 p.m.

Optogenetics aren't just for neuroscientists! In this post, we review optogenetic tools for cytoskeleton and membrane control in cells.

Sept. 28, 2023, 1:15 p.m.

Cas14 was discovered by the Doudna lab. It cleaves ssDNA (but not dsDNA) in trans and can be adapted for a diagnostic toolkit such as DETECTR.

Nov. 29, 2018, 1:53 p.m.

Though microbes are microscopic, there are many ways to visualize microbes. In this blog post, we summarize the latest tools in science communication to showcase microbes - foldscopes, agar art, and GIFs have all been recently use to bring microbiology to diverse audiences.

Aug. 28, 2018, 12:04 p.m.

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

June 28, 2018, 3:01 p.m.

A toolkit of synthetic promoters for transgene expression in AAV can be used for targeted gene expression in retinal cell types in mice, primate, and human retinas.

March 12, 2020, 1:15 p.m.

Find ideas to make your research more environmentally friendly. In this blog post, learn how to you can evaluate products to choose more sustainable ones, reuse plastic, and repurpose regulated medical waste.

March 7, 2019, 2:39 p.m.

Blog editor Tyler Ford runs down some of the best posts from August 2016. NgAgo, site directed mutagenesis, and much more!

Sept. 2, 2016, 1:32 p.m.

Read six spooky science stories and learn about Halloween traditions at Addgene.

Oct. 31, 2019, 1:06 p.m.

The basics of PCR covering end point PCR, qPCR, ddPCR, and multiplex PCR and how each method is used.

Aug. 10, 2021, 1:15 p.m.

Learn about the different types of stem cells in this guide. In particular, we discuss the pros and cons of using embryonic stem cells and adult stem cells and using induced pluripotent stem cells (iPSCs) as a model for regenerative medicine.

Oct. 30, 2018, 1:04 p.m.

Learn about how CRISPR systems are classified. Addgene's guide to typing CRISPR.

March 18, 2025, 1:15 p.m.

Find a list of databases and resources for COVID-19 research and hear from the creators of these databases.

May 12, 2020, 1:15 p.m.

We've made it easier than ever to get support for our resources - Check out the new help center for answers to all of your frequently asked questions.

Dec. 10, 2015, 3:30 p.m.

Overview of recombinases that can be used as a Cre-alternative for site-specific recombination in Drosophila.

May 12, 2015, 1:32 p.m.

Hear from the authors about the generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9.

Feb. 18, 2015, 3:09 p.m.