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We narrowed to 682 results for: plasmid

Showing: 461 - 480 of 682 results

  2. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...procedures. The video details how to transfect CRISPR plasmids into MEL cells by electroporation, sort your transfected... PubMed. More CRISPR Refereces: Browse CRISPR Plasmids CRISPR Information at Addgene More CRISPR-...

  4. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...was to make my own glass milk for purifying cut plasmid DNA from agar slices.  Molecular biologists used...bromide, less stop buffer and miniscule amounts of plasmid sample.  How satisfying?  With practice, I got ...

  5. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...its partner in crime, Cascade. Find Cascade-Cas3 plasmids in Addgene's collection! A “complex” system Cas3... Various labs have designed all-in-one Cascade plasmids — such as the Tomoji Mashimo lab and the Tilmann...resources on Addgene.org CRISPR guide Cascade-Cas3 Plasmid collection ...

  9. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...compared to transgenic animals (17).  These viral plasmids are available through Addgene.  The combination...University of Miami Miller School of Medicine. Find plasmids from the Lee Lab here. Pantelis Tsoulfas is an...University of Miami Miller School of Medicine. Find plasmids from the Tsoulfas lab here.     References: H...

  11. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...for mammalian RNAi Browse all plasmids for RNAi Check out Addgene's plasmids and resources for plant biology...harvested instead of microscopic isolation. The plasmids required to conduct an APT-based RNAi experiment... target sequence, as well as essential control plasmids. Many thanks to our guest blogger Robert Orr...

  12. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...this evolving field! Find prime editing plasmids! Find plasmids from David Liu's lab! This post was originally...the CRISPR guide Find CRISPR plasmids by function Browse our entire plasmid collection ...for treating genetic diseases. In our summer Hot Plasmids post, we covered the Liu lab’s first demonstration...

  13. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...the upstream recombination outcome. Find SPARC plasmids at Addgene To more precisely control the amount...Resources on Addgene.org Find fluorescent protein plasmids at Addgene Read our Molecular Biology Reference...

  14. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...Nathaniel Roquet from the Lu Lab at MIT developed plasmid based systems that report the order in which specific... plasmid...

  15. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...accelerate your research.   Why distribute virus? Plasmid distribution is booming, but, over the years, many...list of what’s available or suggest additional plasmids with the Viral Service Suggestion Form. Lentivirus...

  18. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...you want to give GeoCas9 a try, as always, the plasmids are available from Addgene. Happy CRISPRing! ...Background with Addgene’s CRISPR Guide Page Find CRISPR plasmids at Addgene ...

  19. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ... 12–15 kDa Ease of cloning and expression from plasmids, due to only needing one open reading frame Ability...Membrane Proteins More resources from addgene.org  Plasmids encoding nanobodies at Addgene Sybody Generation...

  20. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    Published
    July 11, 2024, 1:15 p.m.
    ...on the Addgene blog CRISPR 101 eBook en español Plasmids 101 eBook 4th edition Resources on addgene.org...
Showing: 461 - 480 of 682 results