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We narrowed to 689 results for: plasmid

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Showing: 461 - 480 of 689 results

  1. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...recently that researchers began building optimal plasmids to harness the power of Class 1 systems. Class...Lastly, type IV systems are most often found on plasmids. The many unique features of IV-A and IV-B have...led to the hypotheses that they are involved in plasmid competition or can hijack machinery from other ...highly diverse and involved in competition between plasmids. Nucleic Acids Research, 48(4), 2000–2012. https... resources on Addgene.org CRISPR guide CRISPR plasmids CRISPR references ...

  6. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...procedures. The video details how to transfect CRISPR plasmids into MEL cells by electroporation, sort your transfected... PubMed. More CRISPR Refereces: Browse CRISPR Plasmids CRISPR Information at Addgene More CRISPR-...

  8. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...was to make my own glass milk for purifying cut plasmid DNA from agar slices.  Molecular biologists used...bromide, less stop buffer and miniscule amounts of plasmid sample.  How satisfying?  With practice, I got ...

  9. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...its partner in crime, Cascade. Find Cascade-Cas3 plasmids in Addgene's collection! A "complex" system Cas3... Various labs have designed all-in-one Cascade plasmids — such as the Tomoji Mashimo lab and the Tilmann...resources on Addgene.org CRISPR guide Cascade-Cas3 Plasmid collection ...

  13. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...compared to transgenic animals (17).  These viral plasmids are available through Addgene.  The combination...University of Miami Miller School of Medicine. Find plasmids from the Lee Lab here. Pantelis Tsoulfas is an...University of Miami Miller School of Medicine. Find plasmids from the Tsoulfas lab here.     References: H...

  14. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...for mammalian RNAi Browse all plasmids for RNAi Check out Addgene's plasmids and resources for plant biology...harvested instead of microscopic isolation. The plasmids required to conduct an APT-based RNAi experiment... target sequence, as well as essential control plasmids. Many thanks to our guest blogger Robert Orr...

  16. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...this evolving field! Find prime editing plasmids! Find plasmids from David Liu's lab! This post was originally...the CRISPR guide Find CRISPR plasmids by function Browse our entire plasmid collection ...for treating genetic diseases. In our summer Hot Plasmids post, we covered the Liu lab’s first demonstration...

  17. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...the upstream recombination outcome. Find SPARC plasmids at Addgene To more precisely control the amount...Resources on Addgene.org Find fluorescent protein plasmids at Addgene Read our Molecular Biology Reference...

  20. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...you want to give GeoCas9 a try, as always, the plasmids are available from Addgene. Happy CRISPRing! ...Background with Addgene’s CRISPR Guide Page Find CRISPR plasmids at Addgene ...
Showing: 461 - 480 of 689 results