We narrowed to 625 results for: Dos

1. New Tool for Lineage Tracing: The ClonTracer Library

   Type

   Blog Post

   Published

   Sept. 22, 2015, 2:30 p.m.

   ...and Validation Group and says that she decided to do a post-doc at Novartis because she wanted first-hand...

2. CRISPR 101: Non-Homologous End Joining

   Type

   Blog Post

   Published

   April 16, 2015, 3:45 p.m.

   ... ends better substrates for ligation. Although we do not describe these steps here, the processing of ...

3. Plasmids 101: Cre-lox

   Type

   Blog Post

   Published

   Jan. 13, 2015, 3:47 p.m.

   ...Have you used the Cre-lox system in your research? Do you have any tips or tricks, or clever cre-dependent...

4. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   Published

   Nov. 5, 2019, 1:28 p.m.

   ...we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment? The three years...

5. RaPID Detection of RNA-protein Interactions

   Type

   Blog Post

   Published

   Aug. 16, 2018, 12:40 p.m.

   ...RaPID can be used to study RNA-protein interactions. Do you have a question that RaPID could help answer?...

6. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

   Type

   Blog Post

   Published

   June 26, 2018, 1:33 p.m.

   ...References: Monticelli, M., Ferro, T., Jaeken, J., dos Reis Ferreira, V. & Videira, P. A. Immunological ...

7. Human Germline Editing Using CRISPR

   Type

   Blog Post

   Published

   Aug. 10, 2017, 2:19 p.m.

   ...diagnosis (PGD) screens embryos to find those that do not carry a given dominant disease-causing gene, ...

8. Multiplex Genome Editing with CRISPR-Cpf1

   Type

   Blog Post

   Published

   May 9, 2017, 2:12 p.m.

   ...: A New Tool for CRISPR Genome Editing Which Cas9 Do I Choose for My CRISPR Experiment A Match Made in...

9. Plasmids 101: FLEx Vectors

   Type

   Blog Post

   Published

   April 28, 2016, 2:30 p.m.

   ...off"). These Cre-off switches may be referred to as DO (Double-floxed Orientation) and could be used to ...

10. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type

    Blog Post

    Published

    Dec. 16, 2015, 3:30 p.m.

    ...strand breaks (BLESS), revealed that these mutants do not cause off-target effects at unanticipated sites...

11. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type

    Blog Post

    Published

    Dec. 8, 2022, 2:15 p.m.

    ...resources on the Addgene Blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? The PAM requirement...

12. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type

    Blog Post

    Published

    Nov. 3, 2020, 2:15 p.m.

    ...H134R) both reach the brain, but individual neurons do not express as many copies of the ChRs as compared...

13. Addgene: The First Twenty Years

    Type

    Blog Post

    Published

    Dec. 19, 2024, 2:15 p.m.

    ...invaluable resource. I really appreciate the work you do to make sharing reagents and protocols so easy!” ...

14. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type

    Blog Post

    Published

    Aug. 6, 2019, 12:55 p.m.

    ...colony of genetically engineered mice, what can you do? In this post, we’ll cover why and how to cross mice...

15. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type

    Blog Post

    Published

    May 5, 2015, 3:54 p.m.

    ...Writing Scientific Manuscripts by Johnna Roose Dos & Don'ts When Publishing a Scientific Manuscript ...

16. Antibodies 101: Conjugation

    Type

    Blog Post

    Published

    March 26, 2024, 1:15 p.m.

    ... unconjugated version, but if you find yourself doing your own conjugation, it is important to keep in...

17. 5 Steps to Writing Grant Proposals for Grad Students

    Type

    Blog Post

    Published

    Feb. 11, 2014, 12:13 p.m.

    ...application process? What types of writing feedback do you find most helpful? Have a great grant writing...

18. Overcoming the Challenges of Lentiviral Production

    Type

    Blog Post

    Published

    Aug. 7, 2018, 12:31 p.m.

    ... provided you with a few ideas to consider if you do run into a roadblock and encourage you to check out...overcome this issue, we recommend using a range of doses of lentivirus when transducing a target cell line...transduction experiment. Media choice, reaction vessel, dose of virus, volume and timing of transduction will...

19. Plasmids 101: Protein Expression

    Type

    Blog Post

    Published

    June 7, 2018, 1:17 p.m.

    ...Despite their advantages, mammalian expressions systems do require demanding cell culture conditions compared...

20. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type

    Blog Post

    Published

    May 24, 2018, 1:20 p.m.

    ...after knockdown of beta-catenin. Other gene targets do not require amputation to be visible. For example...