We narrowed to 84 results for: control gRNA

Addgene reached its 15 year anniversary on January 8, 2019. In this blog post, find the top 15 requested plasmids since our founding. Many of these include lentiviral or CRISPR tools!

Jan. 8, 2019, 1:54 p.m.

Here, we cover four different plasmid screening strategies including blue-white screening, colony PCR, restriction digest analysis, and Sanger sequencing.

Sept. 15, 2020, 1:15 p.m.

Need help with the plasmid deposit process at Addgene? Consult this post for an in-depth walk through the entire process.

Feb. 27, 2024, 2:15 p.m.

X-CHIME, a series of CRISPR tools developed and deposited by Arlene Sharpe's lab, allows for germline knockouts in immune cells in mice.

Jan. 16, 2024, 6:13 p.m.

Addgene's new viral service provides ready-to-use viral preps for your research.

Nov. 17, 2016, 3:30 p.m.

With many different Cas9 variants out there, it can be hard to know which one to use in your particular CRISPR experiment. This post is here to help.

Oct. 13, 2022, 1:45 p.m.

A round up of guest blog posts of 2024. Thank you to our guest contributors!

Nov. 26, 2024, 2:15 p.m.

Studying the biophysics of Cas9 DNA interaction, the Corn lab has developed a means to enhance CRISPR genome editing through homology directed repair.

March 24, 2016, 2:30 p.m.

iTOP is a protein-only transduction method for delivering proteins and RNA into a variety of cell types, with potential applications with CRISPR/Cas9.

June 23, 2015, 8:37 p.m.

Learn how you can use HDR and CRISPR to make a knock-in cell line for your research.

Dec. 22, 2022, 2:15 p.m.

22 of the interesting and popular hot plasmid technologies of 2014 from Addgene's plasmid repository.

Jan. 6, 2015, 5:21 p.m.

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

March 13, 2014, 4:08 p.m.

Check out all of the plasmids featured in our hot plasmids articles from 2017.

Jan. 9, 2018, 3:02 p.m.

Learn about the basic elements that make up the AAV transfer plasmid, including the ITRs, transgene, promoter, and other cis-regulatory elements.

Aug. 11, 2020, 1:15 p.m.

A new CRISPR/Cas9 system allows genome editing in all E. coli strains.

April 13, 2021, 1:15 p.m.

This post highlights ways to overcome the AAV size limitation to deliver CRISPR editing tools to cells.

Sept. 16, 2020, 1 p.m.

Use the deposit spreadsheet to quickly deposit multiple plasmids with Addgene.

March 8, 2017, 3:15 p.m.

Curious about what a virus is, and how it differs from a viral vector? Check out our Viral Vectors 101 blog that explains it all!

Aug. 18, 2023, 4:15 p.m.

This round-up of new plasmids from Addgene include blue-light inducible Cre for use in bacteria, CRISPR-Casɸ mGold, and new CRISPR plasmids and viral preps.

Jan. 5, 2021, 2:15 p.m.

With the addition of ABE8, a faster and improved adenine base editor, scientists can now make base edits more efficiently and in more contexts.

March 17, 2020, 1:32 p.m.