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Gibson Assembly is a popular cloning alternative to traditional restriction/ligation methods. Learn more about this technique here.

March 1, 2016, 3:30 p.m.

An overview of the Brainbow system and its variants and how they utilize Cre-lox for multicolor labeling of neurons.

April 24, 2015, 2:39 p.m.

Learn about hot new tools in our collection!

Feb. 17, 2026, 2:15 p.m.

Learn more about NanoBiT technology and explore examples of HiBiT-tagged fusion proteins in action.

Oct. 2, 2025, 1:15 p.m.

Learn how repressible promoters work and how you can use them in your next experiment.

Dec. 20, 2022, 2:15 p.m.

Learn about the many fluorescent protein tools enabled by the nanoLuc luciferase.

Feb. 8, 2018, 12:17 p.m.

Learn what non-model organisms are, why scientists use them, and what they can teach us.

July 21, 2026, 1:15 p.m.

Get the basics on RNA interference (RNAi), how it compares with CRISPR, and tools for RNAi studies in plants.

Oct. 27, 2020, 1:15 p.m.

Lambda Red mediated recombineering can be used to generate point mutations in plasmids and bacterial genomes. Learn how to use Lambda Red in this post.

Dec. 15, 2016, 3:57 p.m.

Learn about the process to select the optimal serotype, promoter, and titer for your AAV experiment.

June 1, 2023, 1:15 p.m.

Get tips on choosing the best fluorescence microscopy technique for your next imaging experiment.

Oct. 10, 2017, 1:57 p.m.

Ready to let your tags do the work for you? Learn about self-labelling protein tags and how they can give you flexibility in your experimental approach!

April 16, 2024, 1:15 p.m.

Learn about how CRISPR systems are classified. Addgene's guide to typing CRISPR.

March 18, 2025, 1:15 p.m.

Learn about the many ways you can deliver reprogramming factors to generate induced pluripotent stem cells (IPSCs).

April 17, 2018, 1:37 p.m.

Check out all of the plasmids featured in our hot plasmids articles from 2016.

Dec. 22, 2016, 3:03 p.m.

Check out all of the plasmids featured in our hot plasmids articles from 2017.

Jan. 9, 2018, 3:02 p.m.

22 of the interesting and popular hot plasmid technologies of 2014 from Addgene's plasmid repository.

Jan. 6, 2015, 5:21 p.m.