960 results

Addgene and Seeding Labs recently awarded plasmids to two researchers in Ghana. Read about their work inside.

June 15, 2016, 1:30 p.m.

We've recently curated a Microbiology Plasmid Collection. Learn what you can find in the collection in this post.

June 13, 2016, 7:08 p.m.

In this post we directly compare the Argonaute genome editing protein, NgAgo, to the CRISPR endonuclease, Cas9.

June 9, 2016, 2:30 p.m.

Guest blogger Kate Palozola gives some tips on how to keep a good and organized lab notebook for bioinformatic analyses.

June 2, 2016, 2:30 p.m.

Vectors containing the piggyBac transposon are now available at Addgene. Learn how these unique tools can be used for genome engineering!

May 31, 2016, 3:30 p.m.

Not getting enough DNA from your plasmid preps? This post outlines variables like endA and low copy number that could affect DNA yields and suggests steps to super-charge your plasmid preps.

May 26, 2016, 2:30 p.m.

In this podcast, learn about Kingdom Kwapata, a Malawian researcher currently developing transgenic crops and new means to produce biofuels.

May 24, 2016, 2:30 p.m.

Lentiviruses are useful for delivering DNA sequences to non-dividing mammalian cells. Learn all about the components of lentiviral vectors inside.

May 19, 2016, 2:30 p.m.

Colony PCR is a useful technique for quickly screening for successful constructs after a plasmid cloning experiment.

May 12, 2016, 2:30 p.m.

Scientists are currently developing disease treatments using CRISPR. Learn about recent developments in the fight against HIV-1 with CRISPR.

May 10, 2016, 2:30 p.m.

Cell culture can be difficult work, follow these tips to keep your cell lines free of contamination.

May 5, 2016, 2:30 p.m.

Antibiotic resistant bacteria are a huge issue in the clinic - learn how CRISPR is being developed to fight against antibiotic resistant super bugs.

May 3, 2016, 2:30 p.m.

FLEx vectors utilize Cre/Lox to conditionally manipulate gene expression in vivo.

April 28, 2016, 2:30 p.m.

Many commonly used fluorescent proteins form dimers producing artifacts in a variety of experimental settings. Learn how to avoid these artifacts.

April 19, 2016, 2:30 p.m.

R bodies are tiny, tunable molecule machines that can be used to break membranes. Learn all about them and their potential uses here.

April 14, 2016, 2:30 p.m.

The CombiGEM technology allows a researcher to design and screen CRISPR gRNA libraries for effects on your biological phenomenon of choice.

April 12, 2016, 2:30 p.m.

Lentiviral transductions can be frustrating. Follow these tips to achieve consistently successful lentiviral transductions.

April 7, 2016, 2:30 p.m.

Addgene is teaming up with nonprofit Seeding Labs to provide plasmids to researchers in developing countries.

April 5, 2016, 2:30 p.m.

This post provides an overview on how transcription terminates in prokaryotes and eukaryotes.

March 31, 2016, 2:30 p.m.

PCR can be used to append the appropriate restriction sites to your insert and facilitate downstream cloning. Read on for tips on this process.

March 29, 2016, 2:30 p.m.