960 results

Studying the biophysics of Cas9 DNA interaction, the Corn lab has developed a means to enhance CRISPR genome editing through homology directed repair.

March 24, 2016, 2:30 p.m.

Perturb the activity of 17 different signaling pathways using the cancer pathways ORFs kit.

March 22, 2016, 2:30 p.m.

Viral minigenomes provide a safe way to study dangerous viruses like Ebola. Read all about minigenomes inside.

March 17, 2016, 2:30 p.m.

Can't find a plasmid at Addgene? Suggest new plasmids for the repository by tweeting at us of filling out our "Suggest A Plasmid" form here.

March 16, 2016, 5:30 p.m.

It's important to determine how much functional virus you have a in your prep before starting a transduction. Learn how to titer your lentivirus here.

March 15, 2016, 2:30 p.m.

Learn how Addgene works with you to increase your work's visibility and citations through efforts to organize and curate the repository.

March 10, 2016, 3:30 p.m.

We're recruiting contributors to the Addgene Blog to expand the educational reach of our Plasmids 101 series. Sign up to contribute!

March 2, 2016, 3 p.m.

Gibson Assembly is a popular cloning alternative to traditional restriction/ligation methods. Learn more about this technique here.

March 1, 2016, 3:30 p.m.

Learn about the founding the science communication nonprofit (CINSO) and get tips on thinking about your science career.

Feb. 25, 2016, 3:30 p.m.

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

Feb. 23, 2016, 3:30 p.m.

Restriction cloning is the cloning technique most molecular biologists start off with. Learn the ins and outs of restriction cloning here.

Feb. 18, 2016, 3:42 p.m.

REPLACR-mutagenesis is a new in vivo method for mutating your plasmid constructs. Learn how REPLACR stacks up against other mutagenesis techniques.

Feb. 10, 2016, 3:30 p.m.

Tired of meetings? Read this blog post for tips on how to run an enjoyable and productive meeting.

Feb. 9, 2016, 3:30 p.m.

Learn about the components of a plasmid page on our website and shows how the different components can be useful to you

Feb. 4, 2016, 3:30 p.m.

A quick guide on the main components of CRISPR: the Cas9 endonuclease and the guide RNA.

Feb. 2, 2016, 5 p.m.

Three studies recently showed that CRISPR can be used to treat Duchenne muscular dystrophy in mice. Learn about the work and obstacles to application.

Jan. 26, 2016, 3:30 p.m.

Dr. Michael Koeris is cofounder Biotech startup, Sample 6, and a member of the Addgene Board. Listen to his journey from grad student to entrepreneur.

Jan. 21, 2016, 4:52 p.m.

Find tips on how to perform both large and small scale plasmid verification and sequencing.

Jan. 14, 2016, 3:30 p.m.

Addgenie Emma Markham describes her winding career path and provides advice on how you can find a great job after undergrad.

Jan. 12, 2016, 3:30 p.m.

The Addgene repository has many amazing plasmids used by labs all over the world, but what plasmid was request most in 2015?

Dec. 30, 2015, 3:30 p.m.