We narrowed to 236 results for: cloning

Multicistronic or bicistronic vectors contain IRES and/or 2A peptides to allow for coexpression of multiple genes from a single transcript.

Sept. 9, 2014, 8:20 p.m.

Learn how you can use planarian plasmids from the Sánchez lab to teach developmental biology techniques to new molecular biologists.

May 24, 2018, 1:20 p.m.

Check out all of the plasmids featured in our hot plasmids articles from 2016.

Dec. 22, 2016, 3:03 p.m.

Learn about the different ways you can introduce DNA or RNA into cells using methods such as transformation, transduction, conjugation, and transfection.

June 25, 2019, 12:54 p.m.

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

March 13, 2014, 4:08 p.m.

Learn how to produce, purify, and titer adenoviral vectors for your research.

June 14, 2018, 11:44 a.m.

Learn what a high fidelity DNA ligase is and when you might need one.

March 6, 2018, 1:49 p.m.

Read on for a sampling of some of the exciting new plasmid technologies that have come through our doors in 2015.

Dec. 23, 2015, 3:30 p.m.

Interested in sharing your lab protocols with others? Here are 7 software apps that can help you share and edit your protocols with other scientists.

Sept. 23, 2014, 3:05 p.m.

Learn how various lab techniques have evolved but still remain relevant in many research arenas today.

June 21, 2016, 2:30 p.m.

A round up of guest blog posts of 2024. Thank you to our guest contributors!

Nov. 26, 2024, 2:15 p.m.

Learn about the latest statistics on CRISPR plasmid deposits and distributions and how this reflects the broader CRISPR community.

Aug. 6, 2026, 7 p.m.

Use plasmids to develop synthetic nanobodies in a few weeks with a three step selection process.

June 18, 2020, 1:15 p.m.

Plasmid incompatibility is the inability of different plasmids to be maintained in a cell. We’ll cover why this happens and how it might affect your experiment.

March 31, 2020, 1:15 p.m.

Gamma-retroviral vectors and lentiviral vectors can be used in a wide variety of experiments including those involving genome engineering, RNAi, and inducible gene expression. In this post, learn about some of the most popular viral vectors available from Addgene and their uses.

July 24, 2018, 12:51 p.m.

Learn about pSiM24, a new binary vector drives gene expression in plants.

Sept. 29, 2015, 2:30 p.m.

Hear from the authors about the generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9.

Feb. 18, 2015, 3:09 p.m.

Learn how to optimize CRISPR-activated homology directed repair to make targeted edits in mammalian cells.

Sept. 5, 2017, 1:58 p.m.

Learn how to use in vitro screening and other tricks to expedite the CRISPR genome editing process in mice.

June 28, 2016, 10:59 a.m.

In this Episode of the Addgene Podcast learn all about the Synthetic Biology research going on in Tom Ellis' lab at Imperial College London.

Nov. 29, 2017, 2 p.m.