We narrowed to 79 results for: donor

1. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

   Type

   Blog Post

   Published

   Feb. 4, 2020, 2:15 p.m.

   ...acceptor in combination with mRuby2 or mScarlet as the donor in HeLa cells.   Find ShadowR for mammalian cell...

2. Fluorescent Proteins 101: Aptamer Fluorophores

   Type

   Blog Post

   Published

   April 11, 2017, 2:30 p.m.

   ...experts on FRET techniques. By combining standard donor/acceptor FRET know-how with aptamers, aptabeacons...

3. Design Tips for Prime Editing

   Type

   Blog Post

   Published

   Jan. 23, 2025, 2:15 p.m.

   ... genome editing without double-strand breaks or donor DNA. Nature, 576(7785), 149–157. https://doi.org...

4. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

   Type

   Blog Post

   Published

   June 25, 2024, 1:15 p.m.

   ... genome editing without double-strand breaks or donor DNA. Nature, 576(7785), 149–157. https://doi.org...

5. Adeno-associated Viruses (AAVs) for Genome Editing

   Type

   Blog Post

   Published

   March 27, 2018, 1:32 p.m.

   ...CRISPR-mediated integration of large gene cassettes using AAV donor vectors." Cell reports 20.3 (2017): 750-756. PubMed...

6. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

   Type

   Blog Post

   Published

   Dec. 14, 2017, 2:08 p.m.

   ...from CD34+ cells across blood drawn from multiple donors with non-integrating episomal vectors. PLoS One...

7. Hot Plasmids Spring 2024

   Type

   Blog Post

   Published

   May 23, 2024, 1:15 p.m.

   ...; v2, vector 2; BD, binding domain; SDS, splice donor site; SAS, splice acceptor site; pA, polyadenylation...

8. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

   Type

   Blog Post

   Published

   April 9, 2019, 1:13 p.m.

   ... Galphai), the C-terminus and N-terminus of the donor fluorescent protein can be truncated to increase...

9. CRISPR-mediated Plant Base Editors

   Type

   Blog Post

   Published

   Jan. 3, 2019, 1:35 p.m.

   ...frequencies and relies on the efficient delivery of donor DNA to facilitate repair. Since the development ...

10. Harnessing Bacterial Toxins for Allelic Exchange

    Type

    Blog Post

    Published

    Aug. 15, 2019, 12:30 p.m.

    ...This allows conjugation between a pir-containing donor and the recipient of interest. Because pDS132 cannot...

11. Plasmids 101: Restriction Cloning

    Type

    Blog Post

    Published

    Feb. 18, 2016, 3:42 p.m.

    ... Set up restriction digests for your insert (or donor plasmid) and plasmid backbone. Because you lose ...

12. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type

    Blog Post

    Published

    Oct. 11, 2016, 2:30 p.m.

    ...strand break (DSB) formation and do not require donor DNA, . These precise edits tend to be more efficient...

13. A History of Genome Engineering in Popular Culture

    Type

    Blog Post

    Published

    Feb. 25, 2020, 2:15 p.m.

    ... genome editing without double-strand breaks or donor DNA. Nature 576:149–157 . https://doi.org/10.1038...

14. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type

    Blog Post

    Published

    March 21, 2024, 1:15 p.m.

    ...Manufacturers will typically recommend specific fluorescent donors and acceptors in their product descriptions, or...

15. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    Published

    Nov. 6, 2018, 1:12 p.m.

    ...is to use cells that can be easily isolated from donors. Apart from fibroblasts, human keratinocytes from...

16. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type

    Blog Post

    Published

    Sept. 28, 2020, noon

    ...crRNA, (3) three transposase proteins, and (4) the donor DNA. By using a multi-spacer CRISPR array, CRISPR-transposons...

17. 27 Hot Plasmids from 2016

    Type

    Blog Post

    Published

    Dec. 22, 2016, 3:03 p.m.

    ...gene are supplied to the reaction from a prebuilt donor plasmid library, available from Addgene. Site-specific...available). Second, they co-transfected short-arm donor vectors containing mAID and either Neo or Hygro ...

18. 28 Hot Plasmid Technologies from 2015

    Type

    Blog Post

    Published

    Dec. 23, 2015, 3:30 p.m.

    ... between a hook protein stably localized in the donor compartment (e.g. the ER) and a reporter protein...

19. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    Published

    Feb. 28, 2017, 3:30 p.m.

    ... be expressed in a single vector, pCRISPRainbow-DONOR1, with dCas9 supplied by a separate vector. ...