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Showing: 61 - 80 of 759 results

  1. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...in synaptic communication and can trigger other forms of neuronal signaling and regulation. To create ...PDGFR transmembrane domain, followed by a modified form of the cytosolic C-terminal domain of Stargazin ...resulting fusion protein binds to glutamate, conformational changes lead to an increase in fluorescence...soluble protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal expression. CAG-FLEX: ...

  2. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...tuberculosis. Investigation of multispecies biofilm formed by Pseudomonas spp. and Klebsiella spp. Dr. Devarshi...examine the antibiotic susceptibility of biofilms formed on various structured surfaces. Plasmids expressing...recombination-mediated genetic engineering (find more information on bacterial engineering using CRISPR on our ...spp. The effects of these deletions on biofilm formation and growth will then be measured to link the deleted...deleted genes to specific functions in biofilm formation. The quest for plant compounds to treat complications...diabetes will be expressed from plasmids (find more information on bacterial expression systems here) and the...understanding neuronal networks, their interactions and information processing during this condition is crucial. ...

  3. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...hope you’ll give us your input by filling out this form here linked below. We’ll keep the poll open until...

  4. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...site are recognized and bound by a Cre protein, forming a dimer. The two loxP sites then align in a parallel...parallel orientation, allowing the four Cre proteins to form a tetramer. A double-strand DNA break occurs within...the selection marker, Cre can be used to easily perform this eviction. Regulated Cre expression – placing...

  5. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...our 'Split RNA' design, where the pre-processing form of crRNA is expressed and a tracrRNA (the helper... from a separate promoter. In the pre-processing form, the crRNA contains 30 nt of the target spacer, ...will be processed (i.e., cleaved) into a shorter form that contains 20 bp of the target spacer, or in ...Chimeric RNA' design (meaning the mature processed form of crRNA is fused chimerically to a mature tracrRNA...isolate single-cell colonies, grow them up, then perform individual genotyping (using Sanger sequencing,.... You could then confirm this purification by performing a genotyping assay (such as Sanger sequencing... 500ng of 293FT genomic DNA as template after performing gDNA purification and have tried conditions with...

  7. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...conversion of non-malignant forms of cancer to invasive and malignant forms and noticed that myoepithelial...microenvironment. This results in increased expression of transforming growth factor-β signaling, possibly inducing...myoepithelial cells for instance. They retard tumor formation by inhibiting cell growth, invasion, and angiogenesis...

  8. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...mutation in the terminal resolution site (TRS) to form a vector genome with wild-type ITRs at the both ...fold into transcriptionally active double-stranded form through intra-molecular annealing. Figure and legend...

  9. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ... prep, treated with the antibiotic, and colony-forming units quantified. While these methods tend to be...straightforward approaches such as the FACS-based or colony-forming unit assays, whereas labs that routinely produce...

  10. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    Published
    Dec. 16, 2015, 3:30 p.m.
    ...non-target strand and a positively charged groove formed by the Cas9 HNH and RuvC nuclease domains 2. Interactions...eSpCas9(1.1) and SpCas9-HF1 each stall in an inactive form after binding to a target site and propose that ...complementarity to the gRNA, they have a higher propensity to reform double helices and decrease Cas9 cutting efficiency...

  11. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...draft is in presentable form, set up a meeting with your advisor to go over format, syntax, and delivery...be helpful to ask your advisor to supply a budget form used in a previously funded grant application to...eligibility information, submission information, selection criteria, and award administration information. Carefully...includes overview information, key dates, funding description, research plan, award information, eligibility...the RFA. The RFA contains all of the important information required for a successful grant application. ...to fund. Using bold, italic, or underline font formats helps these statements stand out from the rest ...grant application" by the NIH Still want more information on the application process? What types of writing...

  12. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 1, 2018, 1:57 p.m.
    ...fragments are near each other, they combine forces to form a functional BirA* enzyme. This type of experiment...the two BirA* fragments complement each other to form a functional BirA* enzyme. When this interaction... is part of a particular protein complex. Not performed in a native cell context. For AP-MS, cells are...

  13. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...2019.   MLV GAG-POL polyprotein Nanoblades are formed when viral structural proteins, such as the MLV...between virions and the cell surface, and also helps form DNA and retroviral-particle or VLPs complexes. The...types of CRISPR cargo including RNPs for indel formation via NHEJ, precise modification with Homology Directed...

  14. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...environments. The genes encoding these functions form the basis of many molecular biology tools! ...sharing F plasmids contain the genes for bacteria to form a sex pilus, a “bridge” of cytoplasm that allows...Horizontal gene transfer can occur naturally through transformation (cellular uptake from the environment), transduction...one popular method scientists use to promote transformation, but it isn’t just for lab specimens — there...plasmid are now used routinely in biotechnology to transform plant cells. Degradative plasmids: microbial hardiness...Plasmids 101: What is a Plasmid? Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection...

  15. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    Published
    May 4, 2021, 1:15 p.m.
    ..., the antibody can be converted into recombinant form through DNA sequencing of the line and subsequent...recombinant monoclonal antibodies as well as other forms of antibody-based (e.g., nanobodies, ScFVs) and ...Andrews et al., 2019). Finally, scientists can perform the entire process of antibody selection and maturation...recombinant antibodies. The selection process is often performed via phage display, yeast display, ribosome display...they are using and any validation experiments performed will be useful in perpetuity.  Second, plasmids...

  16. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...with all your components. Next, consider the best form for those CRISPR components based on your model ...protein and in vitro transcribed gRNA are combined to form a Cas9-gRNA complex and delivered to cells using...Calcium phosphate Cells Transformed cell lines Transformed cell lines Transformed cell lines Cargo Nucleic...a CRISPR forum where you may be able to find information regarding your system of choice. The table below...

  18. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    Published
    Oct. 8, 2019, 12:33 p.m.
    ... you to share your thoughts through our Feedback Form.  Thank you for your participation in this resource...Data Hub is an open platform for scientists using AAV to share practical information about their experiences...long the scientist waited after injection before performing the experiment. Every entry has an accompanying... so that you can see for yourself how the AAV performed in that experimental setup. For example, this ...

  19. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...Widefield Microscopy is the most common and simplest form of fluorescent microscopy. Collimated (non-converging... beams into the sample. These beams interfere to form a single plane of excitation light that is thinner...cleared tissues These samples always require some form of optical sectioning. Point scanning confocal microscopes...contrast technique in modern biology (1). When performed correctly, fluorescence microscopy provides a ...confocal microscopes use parallelization to enhance performance. In spinning disk microscopy, a metal disk containing...be needed. Most commonly these experiments are performed on a fluorescent widefield microscope. Here, excitation... sufficiently thin samples, deconvolution can outperform optical sectioning techniques due to a higher...

  20. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...of the plate, where it immediately spreads out to form a firm monolayer. The cell clones - easily identified...from SH-SY5Y cells, as these cells did not seem to form discrete colonies but constantly migrated away from... could potentially be picked following colony formation in selective medium containing a low percentage...
Showing: 61 - 80 of 759 results