...advantage over viral vectors (~5 kB payload for AAV and ~8 kB payload for lentivirus). Large cargos, such...in viral vectors, but easily fit in a TE. Transposons are also less likely than viral vectors to induce...transposon’s LTRs, similar to when packaging viral vectors.   Let’s take a closer look at three different ... in human cells at rates similar to retroviral vectors. The hyperactive version of the SB transposase,...efficiency in human cells similar to retroviral vectors. The hyperactive PB transposase (hyPB) is ~10-fold...transfer Transposons are an alternative to viral vector delivery of transgenes, like for iPSC reprogramming...

...kit has a focus on infrastructure with shuttle vectors, several delivery systems which can be engineered...engineered into the TUs, and even viral gene silencing vectors. The Plant Parts III kit has a focus on transformation...expand the Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems...Gantner, Johannes, et al. “Peripheral Infrastructure Vectors and an Extended Set of Plant Parts for the Modular....0197185. Chamness, James C., et al. “An Extensible Vector Toolkit and Parts Library for Advanced Engineering...Rapid Design and Production of Diverse Expression Vectors.” ACS Synthetic Biology, vol. 6, no. 7, American...acssynbio.7b00016. McGee, Abby V., et al. Modular Vector Assembly Enables Rapid Assessment of Emerging CRISPR...

...of embryonic stem cells (ESCs) with a lentiviral vector, an attenuated retrovirus in the same family as...cells. Remove this pillar and the ESC dies. Order Lentivirus from Addgene If you’re interested in designing...

...communication. Viral vectors and the nervous system With the advent of viral vector technology, new methods... at Addgene). AAV vectors: A platform for delivering neuronal tracers AAV vectors have been widely used...genome is a single-stranded DNA molecule and AAV vectors are replication deficient (i.e. they cannot easily...). AAV does not have a membrane envelope like lentivirus, but instead is contained by a capsid which is... low immunogenicity and cellular toxicity. AAV vectors can be equipped with strong promoters for high ... is often made Cre-dependent. If Cre-dependent vectors are used in transgenic animals that express Cre...that also express Cre. Another advantage of AAV vectors is that there are well-established protocols for...

...common delivery methods include retrovirus [2], lentivirus [4], adenovirus [5], Sendai virus [6], plasmid...and in vitro methods are similar except that the vectors carrying the transdifferentiation factors are directly... (iPSCs) by temperature-sensitive Sendai virus vectors. Proc Natl Acad Sci U S A, 2011. 108(34): p. 14234...., Human induced pluripotent stem cells free of vector and transgene sequences. Science, 2009. 324(5928...mouse induced pluripotent stem cells without viral vectors. Science, 2008. 322(5903): p. 949-53. PubMed PMID...Differentiate the Stem Cell Field Learn how lentiviral vectors can be used for gene delivery Resources on Addgene.org...Addgene.org Visit our Stem Cell Pages Find Viral Vectors for Your Research Find Plasmids from the Yamanaka...

...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with ...expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT vector ensures...polycistronic lentiviruses. The MuLE toolbox contains a series of building-block vectors that are combined...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate...a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed with the...MXS-chaining kit contains 5 empty chaining vectors plus a destination vector and a collection of building blocks.... Additionally, by using an adapted destination vector with a heterologous promoter outside of the Multisite...

... delivered as a pooled library via a lentiviral vector to cell lines that either stably expressed Cas9...cheat sheet Read our Quick Guide to All Things Lentivirus Resources on Addgene.org Check out this guide...

...help users get started with tools like CRISPR, lentivirus, and optogenetics — and shared the protocols ... CRISPRTwenty Years of Sharing: Addgene's Viral Vector Service...

... existing transposon and vector systems and creating an all-synthetic vector that included only the elements...These vectors and sgRNA libraries expand upon the CRISPR family of plasmids by offering a lentivirus-based... into a destination vector in a predefined order. Finally, the destination vectors are used with Multisite... eight entry vectors can be used for each Multisite Gateway compatible destination vector for a maximum...step, compatible Level 0 vectors are directionally assembled into a Level 1 vector creating a single transcriptional...of interest into the pCoofy vectors, select the appropriate pair of vector and gene primers from Table...homology between the vector and gene primer for SLIC cloning. Amplify the pCoofy vector and the sequence ...

...pitfalls: they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis of...purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA in the lab. Figure...our CRISPR guide pages Find ready-to-use Cas9 lentivirus ...

...and TALENs using integrase-defective lentiviral vectors." Nature biotechnology 33.2 (2015): 175-178. PubMed... for Your Experiments Get Ready-to-Use CRISPR Lentivirus ...