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We narrowed to 149 results for: pcr

Showing: 61 - 80 of 149 results

  1. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...plagued by a steady barrage of problems. Why didn’t my PCR work? Why didn’t my ligation work? Why are there ... being able to figure out what went wrong with a PCR is a pretty niche skill; outside of a lab, where ...

  2. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ... once decided that to teach high school students PCR, they first had to understand hydrogen bonds and ...unlikely to be able to follow a YouTube video on PCR, while a senior lab scientist is likely to be quickly...

  4. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ... Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting a transformant takes...remaining starter culture can be used for colony PCR and glycerol stocks. Dip/drip plants more than once...

  7. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ... use detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect even low levels of contamination...detection, Uphoff et. al. outline a do it yourself PCR-based approach complete with freely available positive...

  9. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...Reaction (PCR) by using a biotinylated primer. Biotin can also be enzymatically incorporated during PCR using...

  10. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...differentiation process. Moreover, a splinkerette-PCR assay may be used to map the specific genetic loci...and D. J. Adams, “A high-throughput splinkerette-PCR method for the isolation and sequencing of retroviral...

  13. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...single-stranded DNA sequence used as an initiation point for PCR amplification or sequencing. Primers can be exploited...

  14. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ... your cloning experiments and tired of having to PCR many colonies to find a successful clone? Addgenie...

  15. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...DNA quality can inhibit important reactions like PCR or restriction digests. Transfection Grade DNA (high...from tissues, blood, virus, or other samples, for PCR and gel cleanup, and for extracting RNA! (That’s ...

  19. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...(nCas9). Similar to mutagenesis with error prone PCR, EvolvR uses an error prone polymerase to introduce...

  20. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...the crosslinking. Finally, purify your DNA with a PCR purification kit or with a phenol-chloroform extraction...through a variety of downstream methods, such as qPCR or next-gen sequencing. ChIP is an incredibly useful...isolated and purified DNA, such as sequencing or qPCR. Yay!  Where’s the challenge in that?  So while ...
Showing: 61 - 80 of 149 results