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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 103 results for: primer

Showing: 61 - 80 of 103 results

  4. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ... Addgene Blog Using virus in your research - a primer for beginners Adenoviral delivery of CRISPR/Cas9...

  10. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...chatting quickly with the postdoc who’s really good at primer design – but first, you have to know what resources...

  14. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...Vectors? Learn about Viral Vector Elements Read Our Primer on Optogenetics    ...

  17. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...epitope tag or fusion protein. If your sequencing primer was chosen to confirm a tag or fusion protein is...

  18. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...expressed is amplified using a gene-specific forward primer containing the desired restriction site (the MCS...has several options) and a pUCX-specific reverse primer containing the missing codons from the antibiotic...if a small contaminating PCR product formed from primer dimers and containing the missing codons from the...resistance gene is inserted. It is important that primers be designed to minimize the possibility of dimer...
Showing: 61 - 80 of 103 results