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We narrowed to 288 results for: promoter

Showing: 61 - 80 of 288 results

  1. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ...scientists Viral prep filter categories Promoters: Select the best promoter for your target cell type Serotype...world, it can be hard to find your favorite gene, promoter, serotype, or a myriad of other things. That’s...plasmid to express ubiquitin under control of a CMV promoter. Starting with two search terms we see there are...

  2. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...enzymatic activity is measured. The effect of promoters or enhancer regions on gene expression can be ...investigate the effect of regulatory elements, such as promoters, enhancers and untranslated regions, or the effect...are cloned into a plasmid upstream of a minimal promoter linked to luciferase. Activation of the pathway...

  3. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ... may differ (e.g., a set of various mammalian promoters), the same cloning procedure can be used to incorporate... in this method include fluorescent proteins, promoters, enhancers, polyadenylation signals, inducible...Table 1). Each construct was flanked with a CMV promoter (to drive high-level expression) and a polyA signal...multiple identical polyadenylation signals or promoters Gibson et al., 2009 MXS-Chaining, BioBricks,...

  4. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ... expressed under promoters like the tet-responsive element (TRE) or the cFos promoter. For example, neurons...inducibly express tagged iPAK4 from different promoters, specific time points appear as fluorescent bands...new way to record and quantify the dynamics of promoter activity in single cells. Find iPAK4 plasmids ...

  5. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...guide RNA disrupting mutations in donor DNA   Promoting HDR events over other edits Like I said earlier...transition out of S phase, so cells stay in an HDR-promoted cycle longer. This approach may have some negative...eLife)! Use a Cas that facilitates HR What if promoting HR was as simple as swapping out your Cas9? It...generates sticky ends upon cutting. Cas12a may promote HDR by generating ssDNA overhangs at the break ...

  6. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...career in science outreach: engaging the public to promote an awareness and understanding of science. Like...spray paint to make a trophy for the winner. We promoted the contest for about two months, and we got one...social media. I gained a concrete example to use to promote future attempts and learned a bit about recruiting...network and graduate school resources daily to promote CINSO events and recruit speakers and participants...

  7. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...you design your donor template to include a T7 promoter upstream of the left homology arm, perform in ...transcription to create your ssDNA. Note that the T7 promoter will not be included in the final ssDNA template...GG); this design has been shown to increase T7 promoter activity. To avoid re-cutting by Cas9 after editing...containing the homology arms on both sides and the T7 promoter on one end. Typically, Easi-CRISPR works best ...Addgene plasmids. Design PCR primers to add T7 promoter-left homology arm and right homology arm to the...

  8. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...induce transcription at CpG regions of various promoters. Other researchers additionally fused a TAL effector...: dCas9-p300 adds H3K27ac marks to histones in promoter and enhancer regions. These marks are associated...Figure 2: dCas9-Tet1 demethylates cytosines at promoters and enhancers. This targeted demethylation is ...Figure 3: dCas9-DNMT3A methylates cytosines at promoters and enhancers. This targeted methylation is associated...-based acetyltransferase activates genes from promoters and enhancers. Nature Biotechnology 33:510–517...

  10. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...mCherry, all under the control of the human synapsin promoter. Purpose of this AAV pAAV-hSyn-DIO-hM4D(Gi)-mCherry... to a particular cell type. The human synapsin promoter further specifies neuron-specific expression of...Kügler S, Kilic E, Bähr M. Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene...

  11. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...400-500 bp cassettes containing the RNA pol III promoter, gRNA and pol III terminator. These relatively...together in a single vector. In addition, the pol III promoter is relatively weak, and low expression of gRNAs...biological systems. Size is also a factor; the pol lll promoter and terminator need be encoded only once, and ...

  12. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...examined a number of important plasmid elements – promoters, origins of replication, protein tags, and antibiotic...Regulated Cre expression – placing Cre downstream of promoters that are active only in certain cell or tissue...Cre under a variety of ubiquitous and regulated promoters, and many loxP-containing alleles have also been...

  13. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...optimism they encapsulate, and the solutions they promote help motivate you to spend a little time advocating...fight is far from over here at home.  The work of promoting and defending the role of science in our democracies... take in abusing climate science skepticism to promote harmful policies that benefit polluting industries.In...marching in and of itself will be enough. IT WON’T! Promoting the incorporation of science in policy, demanding... that uses yeast cells to study how DNA damage promotes cancer. She is also a science communicator, UBC...

  14. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...-inducible promoter, though Khetrapal et al. also demonstrated that the arabinose promoter worked too.... under both rhamnose- and arabinose-inducible promoters. Tight control of toxin expression is absolutely...a rhamnose-based tightly regulated and tunable promoter system." Biotechniques 40.3 (2006): 355-364. PubMed...

  15. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...repeat (LTR) of the HIV-1 genome, which acts as a promoter, and found that SAM targeting of the enhancer ...NF-KB binding sites increased activation of HIV promoter-luciferase constructs. In multiple HIV-1 latent...cells with activated HIV-1, measured via HIV-1 promoter-driven GFP expression. In cell lines that can ...indels may not ablate viral function, but rather promote resistance to CRISPR/Cas9. While these results ...

  16. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...published systematic reviews on an agent used to promote bone growth based on data voluntarily made available...collaborative endeavor guided by aspirations to promote open science, ensure good stewardship of clinical...

  17. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...resources and information about organizations promoting reproducibility in this booklet (which was also...line we may see further adoption of policies that promote reproducible publishing practices. One example ... also hopefully see a shift toward hiring and promotion practices that focus on reproducibility and ensuring... Including descriptions of many organizations promoting reproducibility List of Tools for Reproducibility...

  18. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...new backbones special: Strong expression: A CMV promoter drives robust gene expression in mammalian cells...creation a breeze. Modular by design: Key elements (promoter, tag, polyA site) are flanked by unique restriction... both new and known regulators at the FOS gene promoter and the MYC locus (Figure 5). Figure 5:...identification of heat-shock proteins at the FOS gene promoter using TurboCas, streptavidin immunoprecipitation...

  20. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ... cells where common eukaryotic promoters (such as CMV or EF1A promoters found in many CRISPR plasmids)...transfection or viral transduction     Appropriate promoters must be chosen for both Cas9 and gRNA expression...
Showing: 61 - 80 of 288 results