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We narrowed to 178 results for: antibody

Showing: 81 - 100 of 178 results

  1. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...excited to launch our Antibody Data Hub, an open-access repository for antibody validation data. We’ll...world. That included 2008 pooled libraries, 290 antibodies, 18,359 viral items, and 166,247 plasmids. We... our distributor partners, with the launch of antibodies in China and expansion of viral services available... in which Addgene will distribute recombinant antibodies (rAbs) developed at IPI. The first IPI collection...

  2. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...Addgene.org Addgene's Antibody GuideAddgene's collection of ready-to-use recombinant antibodies... blog Antibodies 101:Selecting the Right AntibodyAntibodies 101: Secondary AntibodiesAntibodies 101: Buffers...is critical that your controls and your sample antibodies are from the same batch, to avoid unmixing issues...unmixing if your sample is stained with a PE/Cy7 antibody from a batch that is not the same as your single...contain a mixture of beads that will either bind any antibody (positive population) or won’t bind anything (...sometimes distort the fluorescent signature upon antibody binding, leading to discrepancies during unmixing...

  3. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...serotypes, or hundreds of Data Hub reports detailing antibody experimental results. We also redesigned our website...growing collection of thoroughly characterized antibodies, and our contributions to key research papers...papers are already inspiring higher standards in antibody use (Ayoubi et al., 2024; Kahn et al., 2024). Building... Laflamme, C. (2024). A consensus platform for antibody characterization. Nature Protocols. https://doi.org..., A. R. M., Cable, H., & Longworth, S. (2024). Antibody characterization is critical to enhance reproducibility...

  4. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...probably also very interested in having high-quality antibodies and reagents that you know work…preferably before...not alone – we’re all looking for high-quality antibodies and reagents that work for our experiments!) ... the Human Biomolecular Atlas Program (HuBMAP) antibody metadata standards. And the dataset is rich....2018488117 More resources on the Addgene blog Antibodies 101: Multiplex Immunofluorescence Negative Can...Can Be Positive: Open AAV Data with Addgene Antibodies 101: Choosing the Right Isotype...

  5. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...Addgene blog Antibodies 101: Flow Cytometry  Antibodies 101: Reading a Flow Plot Antibodies 101: Conventional...of two stains, in this case through conjugated antibodies targeting the cell surface markers CD11 (anti-mouse...

  6. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    Published
    April 23, 2018, 2 p.m.
    ...customizable protein degradation. Enter TRIM-Away - an antibody-based system developed by the James and Schuh ...protein variants when used with a variant-specific antibody. TRIM21, an E3 ubiquitin ligase, is the basis ...recruits ubiquitin and the proteasome to destroy antibody-bound pathogens. Clift et al. found that addition...addition of exogenous TRIM21 protein along with an antibody targeting the protein of interest led to rapid...co-microinjection of TRIM21 protein and the target antibody works well. In larger cell populations, you can...-Away, and you only need to deliver the target antibody. TRIM-Away reagents and reporters are available...

  7. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...housekeeping proteins” and are detected with an antibodies, just like the POI. This approach is so common...influenced by sample concentration as well as by antibody dilution — both factors we’ve covered in our previous....2013.05.027 Additional Resources on the Addgene Blog Antibodies 101: The Basics of Western Blotting Technical...

  8. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ... were more resistant to neutralizing anti-AAV antibodies. This is particularly useful for therapeutic ...applications in vivo where endogenous anti-AAV antibodies often neutralize the therapeutic efficacy of ...particles with membrane vesicles protects them from antibody recognition and inactivation (György et al., 2014...more resistant to inactivation through anti-AAV antibodies. References György, Bence, et al. "Naturally...enveloped AAV vectors for shielding neutralizing antibodies and robust gene delivery in vivo." Biomaterials...

  9. New Coomassie Protocol Video

    Type
    Blog Post
    Published
    Oct. 31, 2023, 1:15 p.m.
    ... a fast and inexpensive way to determine your antibody's purity? Check out our latest protocol video, ..., "Coomassie Purity Stain of Recombinant Antibodies."  Hot tip! This protocol can easily be adapted for...

  10. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...on the Addgene blog Antibodies 101: Introduction to Immunofluorescence  Antibodies 101: Multiplex Immunofluorescence...ExM protocols are compatible with conventional antibodies, chemical dyes, and genetically encoded fluorescent...microscopy using fluorescent Fab fragment secondary antibodies. Methodsx 9:101796. https://doi.org/10.1016/j.mex...labeled using standard fluorescent proteins and antibodies. Nat Biotechnol 34:987–992. https://doi.org/10.1038...Immunofluorescence Antibodies 101: Affinity Reagents ...

  11. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...uncharacterized protein, or a protein for which a good antibody has not been developed (and just because your ...your protein has a commercially available antibody, that doesn’t mean it’s a good one), then your first step...depend on your application (see table below). The antibodies available for these tags really are good and ...or DYKDDDK 1 Affinity and Purification Good for antibody-based purification; has inherent enterokinase ...and polar residues improves solubility; good for antibody-based detection SUMO ~100 amino acid protein...GKPIPNPLLGLDST   1.4 Affinity and Purification Good for antibody-based purification Combo and cleavage tags...

  12. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ... single domain antibody fragments typically engineered from heavy-chain only antibodies found in camelids...They possess many advantages over conventional antibodies: they are small (12-15 kDa), stable, highly soluble...still just as specific to their target as regular antibodies. These properties make nanobodies easier to transform...

  13. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...induce hypertonicity display enhanced monoclonal antibody production (Oyaas, 1989; Ozturk and Palsson, 1991...Steve KW, et al. "Substantial overproduction of antibodies by applying osmotic pressure and sodium butyrate...18613082. 3. Oyaas, K., et al. "Hybridoma growth and antibody production under conditions of hyperosmotic stress...osmolarity on hybridoma growth, metabolism, and antibody production." Biotechnology and bioengineering ...

  14. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...are a few of the reasons antibodies may result in a lack of response. Antibodies targeting the tumor-augmenting...chemokine production in the tumor microenvironment. Antibodies that block cell-surface tolerance-producing proteins... Currently the FDA has 12 approved monoclonal antibodies targeting the PD-L1 pathway, VEGF production,...solid as well as blood-based tumors. Monoclonal antibodies have been shown to aide chemotherapy and result...successful elimination of certain tumors. However, antibody stability, half-life, tumor penetration, inadequate... Weiner, George J. "Building better monoclonal antibody-based therapeutics." Nature Reviews Cancer 15.6...Andrew M., Jedd D. Wolchok, and Lloyd J. Old. "Antibody therapy of cancer." Nature Reviews Cancer 12.4...

  15. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    Published
    Feb. 23, 2023, 2:15 p.m.
    ...ready-to-use recombinant antibodies collection, currently composed of over 100 antibodies. Everything in this...which includes both neuroscience and general-use antibodies, is available in both trial- and full-size, with...

  16. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    Published
    June 22, 2021, 1:15 p.m.
    ...on the variants (Garcia-Beltran et al., 2021). Antibodies from individuals who received both doses of the...disease severity and the development of neutralizing antibody responses in patients. They were able to tweak... “In that context, what we showed was that the antibody responses are really not sufficient after one ...Iafrate AJ, Balazs AB (2021) COVID-19-neutralizing antibodies predict disease severity and survival. Cell 184...

  17. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...Regenerative Medicine (CRM), Edinburgh. Most commercial antibodies do not work in pull-down assays: Epitope tagging...protein complexes - from the cell-lysate using antibodies against the target protein. The captured protein...to find (or generate in-house) a pre-validated antibody for each target protein. To study 20,000 human...frustrating. One elegant solution to sidestep the antibody problem is to tag the protein of interest with...immunoprecipitated using a standard tag-specific antibody (Jarvik and Telmer 1998). Indeed, this method ... of interest, and a universal all-purpose “tag antibody” can be used for downstream IP/ChIP assays. Creating...for both the activity of CRISPR reagents and ICC antibody specificity. Derive clonal lines by single-cell...

  18. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...infection. For example, prevalence of neutralizing antibodies against some AAV types, AAV1, 2, 3 and 5 are ...among the human population. These pre-existing antibodies can interfere with virus-cell interactions and...seriously hinder gene therapy outcomes. Anti-AAV antibodies developed by the immune system – patients’ own...own immune systems can develop antibodies that neutralize the AAV vectors used to treat them. Scientists...

  19. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...Addgene's booth? Well, first, Addgenies! We'll have an antibody specialist, Amrita, and a viral vector specialist...tons of information about Addgene's ready-to-use antibodies, viral vectors, and, of course, plasmids! Plus...

  20. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...beads. Nuclei are then incubated with a primary antibody against the protein of interest. The Protein A...Immunoglobulin G (IgG) thus targeting MNase to antibody bound proteins (Figure 1). Importantly, since ...is helpful to incubate with a rabbit secondary antibody when using a non-rabbit primary, prior to the ...
Showing: 81 - 100 of 178 results