We narrowed to 236 results for: cloning

We've partnered with SnapGene to update our plasmid maps. Learn how to use our new maps to accelerate your research.

June 29, 2017, 1:06 p.m.

The Addgene repository has many amazing plasmids used by labs all over the world, but what plasmid was request most in 2015?

Dec. 30, 2015, 3:30 p.m.

Addgene plasmid pages now show whether a plasmid has been cited in other articles. Use the "References" link to view the articles citing your plasmid.

June 16, 2015, 2:15 p.m.

Fluorescent proteins can mis-fold or form aggregates outside of the cytoplasm leading to errors in localization. Avoid these issues with new mOX-FPs.

Oct. 27, 2015, 3 p.m.

Right variable region, but wrong constant? Chimeric antibodies may be the answer!

April 25, 2023, 1:15 p.m.

Looking for a plasmid that can thrive in a number of different bacteria? Learn about broad host range plasmids in this post!

April 11, 2024, 1:15 p.m.

The pCri plasmids available through Addgene allow researchers to multiplex the protein purification process saving time and frustration.

June 19, 2015, 3:08 p.m.

Learn how you can use the Broad Target Accelerator Plasmid Collections from to help enable precision cancer medicine research.

May 11, 2017, 2:30 p.m.

Learn about the differences in virus production between insect cells and mammalian cells.

Nov. 5, 2024, 8:32 p.m.

Data-driven design of Golden Gate Assembly overhangs greatly increases the number of fragments that can be used in cloning.

Aug. 1, 2023, 1:15 p.m.

Find three ways to purify genomic DNA, plasmid DNA, or PCR products without a commercial kit. Also, find a method for regenerating silica columns for reuse.

Feb. 11, 2020, 2:15 p.m.

Learn about the many ways you can deliver reprogramming factors to generate induced pluripotent stem cells (IPSCs).

April 17, 2018, 1:37 p.m.

Tris? Tris-EDTA? EB? There's many options for eluting plasmid preps. Find out how elution and storage temperature affects DNA stability and downstream applications.

June 4, 2020, 1:22 p.m.

Addgene is pleased to announce the release of the 4th edition of ours Plasmids 101 eBook!

Sept. 5, 2023, 1:15 p.m.

Plasmid-encoded antibodies offers many advantages over other types of antibodies. Learn about how recombinant antibodies are made.

May 4, 2021, 1:15 p.m.

Learn how you can use plasmids to teach students about functional genomics with your own "Pathways Over Time" project.

May 22, 2018, 1:38 p.m.

Announcing Addgene's new Intro to the Lab Bench, a collection of protocols, videos, and quizzes to help guide those new to working in the lab.

Sept. 7, 2021, 1:15 p.m.

Recent highlights from Addgene's collection include tools to study SARS-CoV-2 antibody escape, antibodies for aminoacyl tRNA synthetases, and plasmids for generating nanocompartments for yeast bioproduction.

May 18, 2021, 1:15 p.m.

Learn about the components of a plasmid page on our website and shows how the different components can be useful to you

Feb. 4, 2016, 3:30 p.m.

Practical advice for using CRISPRs: designing your gRNA and introducing it into cells.

Sept. 24, 2020, 1:15 p.m.