We narrowed to 176 results for: crispr c plasmids

Gibson Assembly is a popular cloning alternative to traditional restriction/ligation methods. Learn more about this technique here.

March 1, 2016, 3:30 p.m.

Antibiotic resistant bacteria are a huge issue in the clinic - learn how CRISPR is being developed to fight against antibiotic resistant super bugs.

May 3, 2016, 2:30 p.m.

Comparing and contrasting Cas9 to the other genome editing-capable Cas family proteins.

Dec. 8, 2022, 2:15 p.m.

An introduction to gene, enhancer, and promoter trapping. Relevant examples of each along with pros and cons of each system.

May 2, 2023, 1 p.m.

If you’re using CRISPR to make a genome edit, how do you know if your experiment was successful? Try using fluorescent CRISPR reporters like SRIRACCHA and GEmCherry2.

May 26, 2020, 1:15 p.m.

Cas14 was discovered by the Doudna lab. It cleaves ssDNA (but not dsDNA) in trans and can be adapted for a diagnostic toolkit such as DETECTR.

Nov. 29, 2018, 1:53 p.m.

Learn about the pros and cons of biased and unbiased CRISPR genotyping methods.

Oct. 4, 2016, 2:30 p.m.

In our latest Hot Plasmids, we feature new systemic capsids, Cas-Page, an anti-FLAG antibody, and more!

Feb. 20, 2024, 2:45 p.m.

Learn the basics of CRISPR ribonucleoprotein (RNP) preparation and delivery for your next gene editing experiment.

Sept. 6, 2018, 12:02 p.m.

Learn how to design a CRISPR screening experiment targeting enhancers.

Aug. 9, 2016, 2:30 p.m.

The Framework for In vivo T cell Screens (FITS) allows for in vivo CRISPR screening in T cells. Learn more about FITS in our blog post.

May 2, 2024, 1:15 p.m.

Learn about new RNA-targeting Cas13d enzymes that average only 2.8 kb in size, are easy to package in low-capacity vectors, have high on-target and low off-target editing rates.

May 3, 2018, 1:48 p.m.

Learn the basics behind generating generating mouse models using CRISPR/Cas9.

July 12, 2016, 2:30 p.m.

Learn how you can use HDR and CRISPR to make a knock-in cell line for your research.

Dec. 22, 2022, 2:15 p.m.

Genome-wide CRISPR/Cas9 screens are a high-throughput systematic approach for identifying genes involved in a biological process. Recently the Bassik Lab at Stanford developed a new genome-wide CRISPR knockout screen to analyze unanswered questions about CRISPR screen design by including safe targeting guides and short gRNAs.

Sept. 13, 2018, 1:55 p.m.

Learn about the different open resources for C. elegans like OpenWorm, microPublication, and 3'UTRome, and find C. elegans tools at Addgene.

July 18, 2019, 12:55 p.m.

The Doudna lab recently described a new, thermostable Cas9 variant, GeoCas9. Learn how you can use GeoCas9 in your next genome engineering experimetn.

Sept. 14, 2017, 12:40 p.m.

Guest blogger Pooran Dewari presents results of an NgAgo survey he gave to some of the first NgAgo users. Find out how NgAgo compares to Cas9 so far.

Aug. 4, 2016, 2:30 p.m.

SapTrap improves CRISPR genomic tagging in C. elegans by offering scientists an easy one tube assembly reaction to generate high-throughput targeting vectors.

Oct. 12, 2017, 2:26 p.m.

Learn about how Cascade-Cas3 can be used as a CRISPR tool to degrade large fragments of DNA.

Feb. 11, 2025, 2:15 p.m.