We narrowed to 128 results for: pcr

Addgene is now using NGS services from seqWell Inc for plasmid validation. Learn how the seqWell sequencing process works here.

April 19, 2017, 3:25 p.m.

This blog post describes a a self-excising cassette that can be used with CRISPR/Cas9 tools in C. elegans to generate genomic modifications.

July 7, 2015, 3:36 p.m.

A basic overview of chromatic immunoprecipitation (ChIP)

March 10, 2022, 3:25 p.m.

Curious about how you can expand the number and type of amino acids available in your experiment? Genetic code expansion can help!

June 15, 2023, 1:15 p.m.

Targeted mutagenesis helps molecular biologists make changes to a DNA sequence at a precise location. Read on to learn more about EvolvR, a CRISPR-Cas9 based targeted mutagenesis method from the Dueber Lab.

Feb. 21, 2019, 1:15 p.m.

Find tips on how to perform both large and small scale plasmid verification and sequencing.

Jan. 14, 2016, 3:30 p.m.

Blue-white screening is a well loved technique that allows scientists to quickly identify recombinant clones for further analysis.

June 4, 2015, 1:03 p.m.

Neil McKenna and Bert O’Malley of the Nuclear Receptor Signaling Atlas (NURSA) provide an introduction to nuclear receptors and more.

July 15, 2014, 4:03 p.m.

Adeno-associated Virus (AAV) is used extensively for in-vivo transgene expression. Learn how to use AAV in this post.

May 30, 2023, 1:15 p.m.

Find tips for working with RNA and simple protocols for extracting RNA without using a kit

March 24, 2020, 1:15 p.m.

Getting start with mouse models? Here, we break down how to cross mice to create double knockout lines and Cre-lox lines.

Aug. 6, 2019, 12:55 p.m.

It's important to determine how much functional virus you have a in your prep before starting a transduction. Learn how to titer your lentivirus here.

March 15, 2016, 2:30 p.m.

Learn about Addgene's QC process and how and why we maintain a high standard of sequence accuracy across our plasmid collection.

May 6, 2025, 1:15 p.m.

Find information on CRISPR mammalian expression systems and delivery methods. Use the table to quickly compare between the methods.

Sept. 24, 2015, 2:30 p.m.

Learn how to produce, purify, and titer adenoviral vectors for your research.

June 14, 2018, 11:44 a.m.

Quickly isolate mammalian cells clones using the agarose cloning method.

Sept. 7, 2017, 12:17 p.m.

Researchers inactivate genes to investigate their function. This article describes techniques/plasmids used to knockout and knock-in specific genes in mammalian cells.

Dec. 1, 2016, 3:30 p.m.

Not getting enough DNA from your plasmid preps? This post outlines variables like endA and low copy number that could affect DNA yields and suggests steps to super-charge your plasmid preps.

May 26, 2016, 2:30 p.m.

Interview with Nicola Patron about her work in plant synthetic biology and her Golden Gate MoClo plasmid kit.

June 12, 2014, 3:30 p.m.

Learn about evaluating DNA quality with Spectophotometry using a Nanodrop

July 1, 2025, 1:15 p.m.