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We narrowed to 288 results for: promoter

Showing: 81 - 100 of 288 results

  1. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ... targeting expression involve tissue specific promoters, AAV serotypes, and the ever-growing variety of...channel, such as the serotonin 3 receptor, will promote depolarization in neurons. Alternatively, combining...anion channel, such as the glycine receptor, will promote hyperpolarization. PSAM ligands The advantage of...

  2. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ... site accepts the sgRNA target sequence for U6 promoter expression and the second site accepts the homology... a direct fusion of the desired gRNA to the U6 promoter, and a second plasmid containing the homology ...regions of homology to the gene of interest to promote homology directed repair after a CRISPR/Cas9 induced...

  3. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...majority of transfer plasmids use the weak viral LTR promoter to drive expression of the gene of interest. Consequently...have a chimeric 5'LTR that includes a CMV or RSV promoter as well as a portion of the 5'LTR. Including a...2nd-generation system because the wildtype 5'LTR promoter requires Tat to function. Third-generation plasmids...

  4. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...are therefore good temporal reporters to measure promoter activity, for example. Useful positive controls...dark after the transformation. Use introns Plant promoters can be recognized or leaky in Agrobacterium. This...of the GUS gene with the commonly used CaMV 35S promoter in Agrobacterium. This can be easily avoided by...

  5. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ... repression of transcription through binding a promoter region to inhibit association of transcriptional...targeting their genomic locus of interest, the promoter region of the transcription factor IRF-1. Immunoprecipitation...lab found a microRNA (miR483) that binds to the promoter of the growth factor IGF2 open reading frame to...

  6. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...under-informed with respect to how different serotypes, promoters, and injection methods are best suited to their...containing an expression cassette with an ubiquitous promoter to express GFP in a group of young rats. We found...other important factors such as what is the best promoter to use, or if and how much the spread of the virus...

  7. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ... actually express your gene of interest? Many promoters are species-specific, so you’ll need to design...However, you do still need a species-compatible promoter for expressing your selectable marker. Otherwise...Plasmids 101: Mammalian Vectors Plasmids 101: The Promoter Region Resources on Addgene.org Collection of...

  8. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...transgene expression. Gene regulatory elements [promoters and enhancers, miRNA target sites, and recombination...over systemic delivery. Otherwise, cell-specific promoters and enhancers (Mich et al., 2021) can help restrict...optogenetic and chemogenetic tools. Dual expression Promoters, miRNAs, enhancers, and switches all take up precious...particular experiment is determined by the capsid and promoter/enhancer strengths in the cell type of interest...candidate regulatory elements such as enhancers and promoters for cell type-specific targeting (Chan et al.,...when paired with appropriate cell type-specific promoters or Cre lines. Spatial transcriptomics suggests...AAV-PHP.eB, when paired with the ubiquitous CAG promoter, is relatively biased toward L5 and inhibitory...

  9. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...hM4D(Gi)-mCherry (CD68 is a  microglia/macrophage promoter) 121539  AAV5  pOTTC1596 - pAAV SYN1 HA-hM3D... GP-AAV-CAG-FLEX-jGCaMP7f-WPRE AAV1 with a CAG promoter exhibits high specificity for Purkinje neurons...

  11. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...expression systems in E. coli. The arabinose-inducible promoter pBAD and a low-copy number origin of replication...integratable cassettes, both with doxycycline inducible promoters (Fig. 2a). The first cassette contains the major...constitutively-expressed mARG plasmids driven by CMV promoters. Labs that are new to the mARG expression are ...

  12. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...dCas9 along with transcriptional repressors to the promoter region of the gene of interest. dCas9-based repressors... and a dCas9-KRAB fusion, both targeted to the promoter region. Cas9 activators include Cas9 fused to ...the Suntag array of factors, all targeted to the promoter region.   Ok, I have a Cas9! What do i do ...

  13. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...downside to the AMA from my end as an organizer/promoter was that I didn’t have the direct link to send...of the AMA. This made it a little difficult to promote the AMA on social media beforehand (I just sent...sent people over to r/Science in the initial promotion as a work around… there’s plenty of great stuff there...

  14. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...extreme self-starters and especially good at self-promoting—No one is going to tell you to pick up the pace...diversity and acceptance can be an issue in getting promoted (especially race and gender) Interpersonal issues...

  17. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...limited by the lack of well characterized inducible promoters. Far removed from the bio-industry’s vision of... the same dCas9 protein. By using an inducible promoter to modulate dCas9 expression, multiple scRNA transcriptional...

  18. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...researchers test many different combinations of promoters, terminators, solubility and purification tags...2016). The variety of constitutive and inducible promoters, linkers, and tags makes EcoFlex a great option...been used to examine the effects of different promoters, terminators, enhancers, and cross-cassette interactions... et al., 2019). Many plasmid elements such as promoters or fluorophores are interchangeable across organisms...

  19. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ... vectors within this kit contain a variety of promoters to facilitate gene expression in bacteria, yeast...plasmids within it contain a variety of different promoters followed by leader sequences (encoding small leader...different genes was predictable based solely on the promoters and Shine-Delgarno sequences used in this so-called...

  20. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...protein whose coding sequence is closest to the promoter will be expressed. This system differs from Brainbow...translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes a mutant YFP that .../FRT recombinase. When placed under different promoters, Brainbow and Flpbow can be used to label distinct...
Showing: 81 - 100 of 288 results