We narrowed to 193 results for: GFP

1. Your Top Requested Plasmid in 2016!

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   Blog Post

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   Jan. 11, 2017, 8:47 p.m.

   ... lines. Alternative forms of this vector contain GFP-tagged and un-tagged versions of SpCas9 and further...

2. Fluorescent Proteins 101: Fluorescent Biosensors

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   May 18, 2017, 2:30 p.m.

   ...biosensors as an example. Many calcium biosensors include GFP fused to calmodulin (CaM) and the chicken myosin ...

3. pSiM24: Simplifying Plant Genetic Engineering

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   Blog Post

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   Sept. 29, 2015, 2:30 p.m.

   ...Arabidopsis, and corn. For both β-glucuronidase (GUS) and GFP reporters, as well as multiple transient and transgenic...

4. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

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   Blog Post

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   July 29, 2014, 6:16 p.m.

   ...from Joachim Goedhart: Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection.  ...sun, from the standard oldies but goodies (e.g., EGFP and YFP) to the new and improved fruit colors (e.g...

5. Tag Your Favorite Yeast Genes with Ease

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   Blog Post

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   Nov. 19, 2013, 2:37 p.m.

   ...either the N- or C-terminus (3xHA, 13xMyc, GST, or GFP). Longtine et al.(3) describe a complimentary set...

6. Quick Guide to All Things Lentivirus

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   March 21, 2017, 2:30 p.m.

   ...containing the gene of interest (pTet-IRES-EGFP, pPRIME-Tet-GFP-FF3) and one with either tTA or rtTA (FUW-M2rtTA...viral titre to the number of cells transduced by a GFP encoded virus, and the reverse transcriptase activity...

7. Antibodies 101: Introduction to Antibodies

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   Blog Post

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   Jan. 19, 2021, 2:15 p.m.

   ...blot showing GFP expression in cells that have been transfected with a plasmid encoding GFP. Actin, a common...

8. CRISPR Challenges: Standardization and Homology Directed Repair

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   Blog Post

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   Feb. 14, 2018, 2:36 p.m.

   ...PubMed Central PMCID: PMC5495063 Kan et al. use a GFP-BFP conversion system to assess precision genome ...

9. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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   Blog Post

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   Dec. 13, 2018, 1:14 p.m.

   ...endocytosis of a fluorescent dye and transduction of GFP-expressing AAV particles we did not observe any difference...

10. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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    Blog Post

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    Aug. 11, 2020, 1:15 p.m.

    ...or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins such as SaCas9,...

11. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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    Blog Post

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    May 21, 2020, 1:15 p.m.

    ...transcription activation systems, such as LexA/p65 or mCD8/GFP, to allow sparse labeling in a variety of fly cell...

12. Plasmids 101: What is a plasmid?

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    Blog Post

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    April 2, 2020, 6:17 p.m.

    ...cell Plasmids 101: Green Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor the level of...

13. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

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    Blog Post

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    July 18, 2017, 12:41 p.m.

    ...using a bacterial expression system such as the pET GFP LIC cloning vector and the pOKD plasmids. The recombinant...

14. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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    Blog Post

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    June 17, 2021, 1:17 p.m.

    ...strain transformed with pBLADE expressing superfolder GFP instead of mCherry under the PBAD promoter. They ...

15. New Norepinephrine Indicators: nLightG and nLightR

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    Blog Post

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    Dec. 12, 2023, 2:15 p.m.

    ...contains circularly permuted green fluorescent protein (GFP), and nLightR uses a red fluorescent protein, mApple...

16. Antibodies 101: Affinity Tags

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    Blog Post

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    Sept. 19, 2023, 1 p.m.

    ... (FLAG, Myc, etc.) and fluorescent protein tags (GFP, mCherry, etc.). Fluorescent tags are primarily used...

17. New Viral Vectors - Winter 2025

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    Blog Post

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    Jan. 28, 2025, noon

    ...serotype (multiple) rAAV-CAG::FLEX-rev:: hM4D-2a-GFP AAV1 Chemogenetics Scott Sternson New viral prep ...Recombinase Ian Wickersham New viral prep pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 AAV2 Recombinase James M. ...Recombinase James M. Wilson New serotype pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 AAVrg Recombinase James M... prep AiP14033: pAAV-AiE0779m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4033) AAV-PHPeB Optogenetics... prep AiP14035: pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035) AAV-PHPeB Optogenetics... prep AiP15140: pAAV-AiE0873m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5140) AAV-PHPeB Optogenetics...

18. Year of the Camelid: Antibody Style

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    Blog Post

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    May 9, 2024, 1:15 p.m.

    ...highlighted back in 2015, where sdAbs were used to create GFP scaffolds for transcriptional activation.  sdAbs ...

19. Hot Plasmids - October 2022

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    Blog Post

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    Oct. 6, 2022, 1:15 p.m.

    ...Addgene’s western blot for the myc-tagged protein GFP-smFP-myc expressed from Plasmid 98926. See the Applications...

20. 10 Great Guest Posts We're Thankful For

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    Blog Post

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    Nov. 18, 2016, 3 p.m.

    ...Favorite Fluorescent Protein Oligomerizes in Cells GFP oligomerization can lead to misleading artefacts ...